A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase
A rapid, sensitive colorimetric assay for the high-throughput screening of transaminases in liquid or solid-phase
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DOI：
10.1039/c5cc06817g
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发表时间：
2015-12-18
影响因子：
4.9
通讯作者：
Hailes, H. C.
中科院分区：
文献类型：
--
作者：
Baud, D.;Ladkau, N.;Hailes, H. C.
一种新的比色法已开发筛选转氨酶使用廉价的胺供体。该分析是敏感的，具有低水平的背景着色，并可用于鉴定和分析对醛和酮底物的转氨酶活性在高通量格式。值得注意的是，它也适用于固相菌落筛选。
A new colorimetric method has been developed to screen transaminases using an inexpensive amine donor. The assay is sensitive, has a low level of background coloration, and can be used to identify and profile transaminase activities against aldehyde and ketone substrates in a high-throughput format. Significantly it is also amendable to solid phase colony screening.