Kinetics of Inactivation of Bacillus subtilis subsp. niger Spores and Staphylococcus albus on Paper by Chlorine Dioxide Gas in an Enclosed Space.
Kinetics of Inactivation of Bacillus subtilis subsp. niger Spores and Staphylococcus albus on Paper by Chlorine Dioxide Gas in an Enclosed Space.
复制标题
枯草芽孢杆菌亚种灭活动力学。
DOI：
10.1128/aem.03940-15
复制
发表时间：
2016
影响因子：
4.4
通讯作者：
Zhang,Zongxing
中科院分区：
文献类型：
--
作者：
Wang,Tao;Wu,Jinhui;Qi,Jiancheng;Hao,Limei;Yi,Ying;Zhang,Zongxing
枯草芽孢杆菌黑孢亚种和白色葡萄球菌是灭活空气传播病原体的典型生物指标。本研究描述并比较了不同气体浓度和相对湿度 (RH) 条件下二氧化氯 (ClO2) 气体灭活 B. subtilis subsp. nigerspores 和 S. albus 的行为。通过一阶模型和Weibull模型确定了不同ClO2气体浓度（1至5毫克/升）下的失活动力学。建立了一个新模型（Weibull-H 模型）来揭示不同相对湿度条件（30% 至 90%）下 ClO2 气体的失活趋势和动力学。结果表明，气体浓度和相对湿度与所选两个指标的失活均呈显着（P<0.05）正相关。高RH（>70%）下灭活效率快速提高。与一阶模型相比，Weibull 和 Weibull-H 模型更适合实验数据，表明营养细菌和孢子在暴露于 ClO2 气体后呈现非线性失活行为。根据建立的模型计算了枯草芽孢杆菌黑孢亚种和白色沙门氏菌达到六对数减少所需的时间。阐明 ClO2 气体灭活枯草芽孢杆菌亚种黑孢子和 S. albus 的动力学将有助于开发提供有效消毒方法的 ClO2 气体处理方法。 重要性 二氧化氯 (ClO2) 气体是一种新型有效的熏蒸剂，具有强氧化能力和广泛的杀菌谱。 ClO2 气体的抗菌功效已在许多先前的研究中得到评估。然而，目前尚无已发表的模型可用于描述不同气体浓度和相对湿度条件下ClO2气体灭活空气中病原体的动力学。本研究建立的一阶模型和威布尔(Weibull-H)模型可以表征和比较枯草芽孢杆菌黑孢子和白葡萄球菌在ClO2气体灭活方面的行为，确定两种选定菌株在不同气体浓度和相对湿度条件下的灭活动力学，并提供实现六对数减少的计算时间。这些结果将有助于确定二氧化氯气体灭活受污染空气和其他环境中空气传播病原体的有效条件，从而防止空气传播疾病的爆发。
Bacillus subtilis subsp.nigerspore and Staphylococcus albus are typical biological indicators for the inactivation of airborne pathogens. The present study characterized and compared the behaviors of B. subtilis subsp.nigerspores and S. albus in regard to inactivation by chlorine dioxide (ClO2) gas under different gas concentrations and relative humidity (RH) conditions. The inactivation kinetics under different ClO2gas concentrations (1 to 5 mg/liter) were determined by first-order and Weibull models. A new model (the Weibull-H model) was established to reveal the inactivation tendency and kinetics for ClO2gas under different RH conditions (30 to 90%). The results showed that both the gas concentration and RH were significantly (P< 0.05) and positively correlated with the inactivation of the two chosen indicators. There was a rapid improvement in the inactivation efficiency under high RH (>70%). Compared with the first-order model, the Weibull and Weibull-H models demonstrated a better fit for the experimental data, indicating nonlinear inactivation behaviors of the vegetative bacteria and spores following exposure to ClO2gas. The times to achieve a six-log reduction of B. subtilis subsp.nigerspore and S. albus were calculated based on the established models. Clarifying the kinetics of inactivation of B. subtilis subsp.nigerspores and S. albus by ClO2gas will allow the development of ClO2gas treatments that provide an effective disinfection method.IMPORTANCEChlorine dioxide (ClO2) gas is a novel and effective fumigation agent with strong oxidization ability and a broad biocidal spectrum. The antimicrobial efficacy of ClO2gas has been evaluated in many previous studies. However, there are presently no published models that can be used to describe the kinetics of inactivation of airborne pathogens by ClO2gas under different gas concentrations and RH conditions. The first-order and Weibull (Weibull-H) models established in this study can characterize and compare the behaviors of Bacillus subtilis subsp.nigerspores and Staphylococcusalbusin regard to inactivation by ClO2gas, determine the kinetics of inactivation of two chosen strains under different conditions of gas concentration and RH, and provide the calculated time to achieve a six-log reduction. These results will be useful to determine effective conditions for ClO2gas to inactivate airborne pathogens in contaminated air and other environments and thus prevent outbreaks of airborne illness.