Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes
Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes
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DOI：
10.1016/j.meatsci.2005.04.022
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发表时间：
2005-09-01
期刊：
影响因子：
7.1
通讯作者：
Lonergan, SM
中科院分区：
文献类型：
--
作者：
Huff-Lonergan, E;Lonergan, SM
不可接受的持水能力每年使肉类行业损失数百万美元。然而，有限的进展已经取得了理解的机制，发展的基础上滴或清洗。很明显，死后早期的事件，包括pH值下降的速度和程度，蛋白质水解，甚至蛋白质氧化是影响肉保持水分的能力的关键。肌肉中的大部分水被截留在细胞的结构中，包括肌原纤维内和肌原纤维外空间;因此，细胞内结构的关键变化影响肌细胞保持水分的能力。随着僵直的进展，肌原纤维中容纳水的空间减少，流体可以被迫进入肌原纤维外空间，在那里它更容易以水滴的形式流失。如果将肌松质连接在一起以及将肌原纤维连接到细胞膜的蛋白质（如结蛋白）不被降解，则在僵直期间发生的肌原纤维的侧向收缩可以传递到整个细胞。细胞骨架蛋白的有限降解可能导致整个肌肉细胞的收缩增加，最终转化为滴水损失。最近的证据表明，钙蛋白酶蛋白酶降解的关键细胞骨架蛋白在确定持水能力发挥作用。这篇综述将集中于肌肉中影响与持水能力相关的结构变化的关键事件。(c)2005爱思唯尔有限公司保留所有权利。
Unacceptable water-holding capacity costs the meat industry millions of dollars annually. However, limited progress has been made toward understanding the mechanisms that underlie the development of drip or purge. It is clear that early postmortem events including rate and extent of pH decline, proteolysis and even protein oxidation are key in influencing the ability of meat to retain moisture. Much of the water in the muscle is entrapped in structures of the cell, including the intra- and extramyofibrillar spaces; therefore, key changes in the intracellular architecture of the cell influence the ability of muscle cells to retain water. As rigor progresses, the space for water to be held in the myofibrils is reduced and fluid can be forced into the extramyofibrillar spaces where it is more easily lost as drip. Lateral shrinkage of the myofibrils occurring during rigor can be transmitted to the entire cell if proteins that link myolibrils together and myofibrils to the cell membrane (such as desmin) are not degraded. Limited degradation of cytoskeletal proteins may result in increased shrinking of the overall muscle cell, which is ultimately translated into drip loss. Recent evidence suggests that degradation of key cytoskeletal proteins by calpain proteinases has a role to play in determining water-holding capacity. This review will focus on key events in muscle that influence structural changes that are associated with water-holding capacity. (c) 2005 Elsevier Ltd. All rights reserved.