The autophagy-related genes BbATG1 and BbATG8 have different functions in differentiation, stress resistance and virulence of mycopathogen Beauveria bassiana.
The autophagy-related genes BbATG1 and BbATG8 have different functions in differentiation, stress resistance and virulence of mycopathogen Beauveria bassiana.
复制标题
自噬相关基因BbATG1和BbATG8在真菌病原白僵菌的分化、抗逆性和毒力方面具有不同的功能
DOI：
10.1038/srep26376
复制
发表时间：
2016-05-20
影响因子：
4.6
通讯作者：
Feng MG
中科院分区：
文献类型：
--
作者：
Ying SH;Liu J;Chu XL;Xie XQ;Feng MG
自噬相关蛋白在真核生物中发挥着显著不同的作用。在昆虫病原真菌球孢白僵菌中，自噬与真菌的生长和发育有关。BbATG1(一种丝氨酸/苏氨酸蛋白激酶)和BbATG8(一种泛素样蛋白)在自噬中具有相似的作用，但在其他过程中具有不同的作用。BbATG1和BbATG8的突变株在饥饿胁迫下抑制了分生孢子的萌发。突变体ΔBbATG8表现出对氧化应激的敏感性增强，而Δ突变体BbATG1则没有。BbATG1和BbATG8在孢子分化中的作用不同。ΔBbATG1和ΔBbATG8突变体的产孢量分别下降了70%和92%，双突变体的产孢量下降了95%。分生孢子产量在ΔBbATG1和ΔBbATG8突变体中分别降低了约90%和50%。一个双突变体的减少与Δ的BbATG1相似。此外，BbATG1和BbATG8都影响分生孢子形成所需的分生蛋白BbCP15p的水平。局部和血腔内注射试验表明，每个自噬缺陷突变体的毒力显著减弱，并显示出局部感染的较大减少。然而，BbATG1和BbATG8对真菌毒力的影响不同。我们的数据表明，这些自噬相关蛋白在真菌胁迫反应、无性发育和毒力方面具有不同的功能。
Autophagy-related proteins play significantly different roles in eukaryotes. In the entomopathogenic fungus Beauveria bassiana, autophagy is associated with fungal growth and development. BbATG1 (a serine/threonine protein kinase) and BbATG8 (a ubiquitin-like protein) have similar roles in autophagy, but different roles in other processes. Disruption mutants of BbATG1 and BbATG8 had impaired conidial germination under starvation stress. The mutant ΔBbATG8 exhibited enhanced sensitivity to oxidative stress, while a ΔBbATG1 mutant did not. BbATG1 and BbATG8 showed different roles in spore differentiation. The blastospore yield was reduced by 70% and 92% in ΔBbATG1 and ΔBbATG8 mutants, respectively and the double mutant had a reduction of 95%. Conidial yield was reduced by approximately 90% and 50% in ΔBbATG1 and ΔBbATG8 mutants, respectively. A double mutant had a reduction similar to ΔBbATG1. Additionally, both BbATG1 and BbATG8 affected the levels of conidial protein BbCP15p required for conidiation. The virulence of each autophagy-deficient mutant was considerably weakened as indicated in topical and intrahemocoel injection assays and showed a greater reduction in topical infection. However, BbATG1 and BbATG8 had different effects on fungal virulence. Our data indicate that these autophagy-related proteins have different functions in fungal stress response, asexual development and virulence.