Effect of β-naphthoflavone on hepatic cytochrome P4501A activity in the scribbled rabbitfish (Siganus spinus) from tropical Indo-Pacific coral reefs.
Effect of β-naphthoflavone on hepatic cytochrome P4501A activity in the scribbled rabbitfish (Siganus spinus) from tropical Indo-Pacific coral reefs.
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β-萘黄酮对来自热带印度太平洋珊瑚礁的潦草兔鱼（Siganus spinus）肝细胞色素 P4501A 活性的影响。
DOI：
10.1007/s10646-012-0969-1
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Biggs,JasonS
中科院分区：
文献类型：
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作者：
Emborski,Carmen;Reyes,Andres;Biggs,JasonS
几类致癌环境有机污染物，包括多环芳烃 (PAH)、多氯联苯 (PCB) 和二恶英，对全世界的水生生态系统产生负面影响。污染物检测通常既困难又昂贵，特别是在处理复杂的混合物和基质时。生物标记是识别可能含有有毒化合物的生物源和不适合娱乐的区域的信息工具。目前，还没有物种建立了用于印度太平洋珊瑚礁有机污染物监测的生物标记。本研究评估了斑纹兔鱼 Siganus spinus（Siganidae）中细胞色素 P4501A (CYP1A) 系统的时间和剂量依赖性诱导，作为这些环境中有机污染物暴露的生物标志物。结果表明，单次腹膜内 (IP) 注射经典芳基烃受体 (AHR) 激动剂 β-萘黄酮 (BNF) 后，棘葡萄球菌肝 CYP1A 酶活性和蛋白质水平会产生剂量和时间依赖性反应。分别使用 7-乙氧基试卤灵-O-脱乙基酶 (EROD) 和非竞争性酶联免疫吸附 (ELISA) 测定法测量，刺棘葡萄球菌肝 CYP1A 蛋白和酶活性在前两天随着剂量的变化而上升，并在 16 天后缓慢恢复到接近正常的水平。这些发现支持使用 S. spinus 的诱导型 CYP1A 系统作为珊瑚鱼暴露于沿海海洋污染的生物标志物。还测量并比较了关岛野生 S. spinus 种群中的基线 CYP1A 表达水平。
Several classes of carcinogenic environmental organic pollutants, including polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and dioxins, negatively affect aquatic ecosystems worldwide. Pollutant detection is often difficult and expensive, especially when dealing with complex mixtures and matrices. Biological markers are informative tools to identify living sources that may harbor toxic compounds and areas unsuitable for recreation. Currently, no species have established biomarkers for organopollutant monitoring in Indo-Pacific coral reefs. This study evaluated the time- and dose-dependent induction of the cytochrome P4501A (CYP1A) system in the scribbled rabbitfish, Siganus spinus (Siganidae), as a biomarker for organic pollutant exposures in these environments. Results indicate that S. spinus hepatic CYP1A enzymatic activity and protein level respond dose-, and time-dependently following a single intraperitoneal (IP) injection of the classic aryl hydrocarbon receptor (AHR) agonist, β-napthoflavone (BNF). S. spinus hepatic CYP1A protein and enzymatic activity rose as function of dose during the first two days and slowly returned to levels close to normal after 16 days, as measured using the 7-ethoxyresorufin-O-deethylase (EROD) and the non-competitive enzyme-linked immunosorbent (ELISA) assays, respectively. These findings support use of the inducible CYP1A system of S. spinus as a biomarker for reef fish exposure to coastal marine pollution. Baseline CYP1A expression levels among Guam’s wild S. spinus populations were also measured and compared.