Molecular paternity determination in captive bonobos and the impact of inbreeding on infant mortality
Molecular paternity determination in captive bonobos and the impact of inbreeding on infant mortality
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圈养倭黑猩猩的分子亲子鉴定以及近亲繁殖对婴儿死亡率的影响
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发表时间：
2008
期刊：
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通讯作者：
Z. Pereboom
中科院分区：
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作者：
S. V. Coillie;Peter Galbusera;A. D. Roeder;W. Schempp;Jeroen M. G. Stevens;Kristin Leus;G. Reinartz;Z. Pereboom
近亲繁殖和遗传多样性的丧失可能会降低适应性，降低种群适应不断变化的环境的潜力。在小种群中，例如在圈养种群或濒危物种种群中，这可能对它们的生存产生相当大的影响。我们调查了近亲繁殖对世界圈养倭黑猩猩种群中婴儿死亡率的影响。使用来自基因型个体的studbook数据和高质量系谱数据的组合，可获得142个圈养出生个体的近亲繁殖信息。为了确定在血统书中未解决的亲子关系，使用Great Ape试剂盒和Powerplant ® 16系统从毛发和血液样品扩增核微卫星DNA。总共，54倭黑猩猩（17个后代和他们假定的父母）在8个四核苷酸重复微卫星位点进行基因分型。近亲繁殖系数计算的每个人的父亲是由任一studbook数据或DNA分析确认。我们发现，与非近交后代相比，近交后代的婴儿死亡率显着更高，这表明近交降低了圈养倭黑猩猩的婴儿存活率。此外，我们认为，在这个圈养人口的近亲繁殖抑郁症的总规模可能被低估。总之，尽管圈养倭黑猩猩的繁殖计划旨在避免近亲繁殖，但近亲个体偶尔也会产生表现出近亲繁殖衰退的后代。然而，目前没有迹象表明这会威胁到倭黑猩猩圈养种群的长期生存。
Inbreeding and the loss of genetic diversity may lower fitness and reduce the potential for a population to adapt to changing environments. In small populations, for example in captive populations or populations of endangered species, this can have considerable consequences for their survival. We investigated the effects of inbreeding on infant mortality in the world captive population of bonobos Pan paniscus. Using a combination of studbook data and high‐quality pedigree data from genotyped individuals, inbreeding information was available for 142 captive‐born individuals. For the determination of paternities that were unresolved in the studbook, nuclear microsatellite DNA was amplified from hair and blood samples using the Great Ape Kit and PowerPlex® 16 System. In total, 54 bonobos (17 offspring and their putative parents) were genotyped at eight tetranucleotide repeat microsatellite loci. Inbreeding coefficients were calculated for each individual for whom paternity was confirmed by either studbook data or DNA analysis. We found significantly higher infant mortality in inbred offspring compared with non‐inbred offspring, suggesting that inbreeding reduces infant survival in captive bonobos. In addition, we argue that the total magnitude of inbreeding depression is probably underestimated in this captive population. In conclusion, even though the breeding programme of captive bonobos is aimed at avoiding inbreeding, closely related individuals do occasionally produce offspring that do show inbreeding depression. There is, however, no indication that this currently threatens the long‐time survival of the captive population of bonobos.