Genetic analysis of organoleptic quality in fresh market tomato. 1. Mapping QTLs for physical and chemical traits
Genetic analysis of organoleptic quality in fresh market tomato. 1. Mapping QTLs for physical and chemical traits
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DOI：
10.1007/s001220051643
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发表时间：
2001-02-01
影响因子：
5.4
通讯作者：
Buret, M
中科院分区：
文献类型：
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作者：
Saliba-Colombani, V;Causse, M;Buret, M
改善感官品质是新鲜市场番茄育种者的一个重要但复杂的目标。对影响感官品质变异的26个性状进行了评价，以了解这一性状的遗传控制。重组自交系（RIL）群体是从具有良好的整体香气强度的樱桃番茄品系和具有普通口味但果实更大的自交系之间的种内杂交开发的。物理性状包括单果重、果径、果色（L、a、B）、硬度和弹性。化学性状包括干物质重、可滴定酸、pH值、可溶性固形物、糖、番茄红素、胡萝卜素和12种香气物质的含量。RILs的大多数性状表现出较大的变异范围，其中许多性状具有超亲性。香气物质之间的相关性与其来源的代谢途径一致。通过简单区间作图和复合区间作图，共检测到81个影响26个性状的显著QTL。它们主要分布在第2、3、4、8、9、11和12号染色体上的少数区域。检测到影响果实质量、直径、色泽和6种香气成分的主效QTL（R-2>30%）。控制相关性状的QTL主要定位在第2染色体上。果实重量和糖含量或干物质重的QTL通常共定位。在第9染色体上检测到一个控制可溶性固形物含量和干物质重的QTL，而在一个没有控制果实重QTL的区域内检测到了这两个QTL。7个香气物质、番茄红素含量和果实颜色的QTL也被共定位。将QTL定位结果与番茄与野生番茄杂交结果进行了比较。
Improving organoleptic quality is an important but complex goal for fresh market tomato breeders. A total of 26 traits involved in organoleptic quality variation were evaluated, in order to understand the genetic control of this characteristic. A recombinant inbred line (RIL) population was developed from an intraspecific cross between a cherry tomato line with a good overall aroma intensity and an inbred line with a common taste but with bigger fruits. Physical traits included fruit weight, diameter, color (L,a,b), firmness and elasticity. Chemical traits were dry matter weight, titratable acidity, pH, and the contents of soluble solids, sugars, lycopene, carotene and 12 aroma volatiles. RILs showed a large range of variation for most of the traits and many of them were transgressive. Some correlations between aroma volatiles were in accordance with the metabolic pathway they originated from. A total of 81 significant QTLs were detected for the 26 traits by simple and composite interval mapping. They were mainly distributed in a few regions on chromosomes 2, 3, 4, 8, 9, 11 and 12. Major QTLs (R-2>30%) were detected for fruit weight, diameter, and color, and for six aroma volatiles. Go-localization of QTLs controlling correlated traits was mainly found on chromosome 2. QTLs for fruit weight and sugar content or dry matter weight were often co-localized. However, a QTL for soluble-solids content and dry matter weight have been detected on chromosome 9 in a region without fruit weight QTLs. QTLs for seven aroma volatiles, lycopene content and fruit color were also co-localized. The QTL localizations were compared with those detected in crosses between Lycopersicon esculentum and wild tomato species.