Aerosol Based Fabrication of Thiol-Capped Gold Nanoparticles and Their Application for Gene Transfection
Aerosol Based Fabrication of Thiol-Capped Gold Nanoparticles and Their Application for Gene Transfection
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DOI：
10.1021/cm300601m
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发表时间：
2012-09-25
影响因子：
8.6
通讯作者：
Roberts, Jeffrey T.
中科院分区：
文献类型：
--
作者：
Byeon, Jeong Hoon;Roberts, Jeffrey T.
在这项研究中，环境火花产生的Au纳米粒子负载氮气与1-己烷乙基醚[CH 3（CH 2）（5）SH]和乙醇（EtOH）的雾化溶液混合。Au纳米颗粒与1-己基乙醇在雾化液滴中反应形成加盖的金纳米颗粒，其尺寸分布被测量为不同体积分数的1-己基乙醇。通过将硫醇浓度从0.1%增加到1.0%（v/v），合并的颗粒（Au-硫醇）的尺寸分布从双峰（显示Au和液滴的单独分布的叠加）改变为单峰（仅显示液滴状分布，其中消除了Au分布）构型。后一种现象归因于Au纳米颗粒定量掺入到雾化颗粒中。细胞活力和转染的测量显示，即使合并的颗粒具有更高的细胞密度，（类似于78%的细胞活力>类似于49%的聚乙烯亚胺，PEI）比壳聚糖（接近96%），基因转染率（2.56 × 10（6）RLU mg（-1））高于壳聚糖（7.63 × 10（4））和PEI（6.84 × 10（5））。
In this study, an ambient-spark-produced-Au-nanoparticle-laden nitrogen gas was mixed with an atomized solution of 1-hexanethiol [CH3(CH2)(5)SH] and ethanol (EtOH). The Au nanoparticles reacted with 1-hexanethiol in the atomized droplets to form capped gold nanoparticles, whose size distributions were measured for varying volumetric fractions of 1-hexanethiol. By increasing the thiol concentration from 0.1% to 1.0% (v/v), the size distribution of merged particles (Au-thiol) was changed from bimodal (showing a superposition of individual distributions of Au and droplets) to unimodal (showing only a droplet-like distribution, with the Au distribution eliminated) configuration. The latter phenomenon is attributed to quantitative incorporation of Au nanoparticles into atomized particles. Measurements of cell viability and transfection revealed that even though the merged particles had a higher cytotwdcity (similar to 78% in cell viability > similar to 49% for polyethyleneimine, PEI) than that did chitosan (similar to 96%), the transfection (2.56 X 10(6) in RLU mg(-1)) of gene was higher than those for chitosan (7.63 x 10(4)) and PEI (6.84 X 10(5)).