Lentivirus Mediating FGF13 Enhances Axon Regeneration after Spinal Cord Injury by Stabilizing Microtubule and Improving Mitochondrial Function
Lentivirus Mediating FGF13 Enhances Axon Regeneration after Spinal Cord Injury by Stabilizing Microtubule and Improving Mitochondrial Function
复制标题
慢病毒介导的 FGF13 通过稳定微管和改善线粒体功能来增强脊髓损伤后的轴突再生。
DOI：
10.1089/neu.2017.5205
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发表时间：
2017-12-07
影响因子：
4.2
通讯作者：
Xiao, Jian
中科院分区：
文献类型：
--
作者：
Li, Jiawei;Wang, Qingqing;Xiao, Jian
成纤维细胞生长因子13(FGF13)是成纤维细胞生长因子家族中的一种非分泌性蛋白，通过稳定微管在皮质神经元发育过程中起着至关重要的作用。在以前的研究中，我们表明微管动力学的调节对于生长锥的启动和促进受损轴突的再生都是有用的。然而，FGF13在脊髓或脊髓损伤(SCI)后的表达和作用仍不明确。在这里，我们展示了FGF13在调节微管动力学和促进脊髓损伤后轴突再生中的作用。应用FGF13不仅在体外促进神经元极化、轴突形成和生长锥的启动，而且还促进脊髓损伤后的功能恢复。此外，我们还发现，原代皮质神经元中FGF13的上调伴随着线粒体功能的增强，线粒体功能是轴突再生所必需的。我们的研究明确了FGF13促进轴突再生的新机制，指出FGF13可能成为治疗脊髓损伤或其他中枢神经系统(CNS)损伤的潜在候选者。
Fibroblast growth factor 13 (FGF13), a nonsecretory protein of the FGF family, plays a crucial role in developing cortical neurons by stabilizing the microtubule. In previous studies, we showed that regulation of microtubule dynamics was instrumental for both growth cone initiation and for promoting regrowth of injured axon. However, the expression and effect of FGF13 in spinal cord or after spinal cord injury (SCI) remains undefined. Here, we demonstrated a role of FGF13 in regulating microtubule dynamics and in enhancing axon regeneration after SCI. Administration of FGF13 not only promoted neuronal polarization, axon formation, and growth cone initiation in vitro, but it also facilitated functional recovery following SCI. In addition, we found that upregulation of FGF13 in primary cortical neurons was accompanied by enhanced mitochondrial function, which is essential for axon regeneration. Our study has defined a novel mechanism underlying the beneficial effect of FGF13 on axon regeneration, pointing out that FGF13 may serve as a potential candidate for treating SCI or other central nervous system (CNS) injury.