Diversity in a chorion multigene family created by tandem duplications and a putative gene-conversion event.
Diversity in a chorion multigene family created by tandem duplications and a putative gene-conversion event.
复制标题
由串联复制和假定的基因转换事件产生的绒毛膜多基因家族的多样性。
DOI：
10.1007/bf02104732
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发表时间：
1984
影响因子：
3.9
通讯作者：
Eickbush,TH
中科院分区：
文献类型：
--
作者：
Rodakis,GC;Lecanidou,R;Eickbush,TH
家蚕高半胱氨酸绒毛膜蛋白的两个家族由15个串联排列的不相同基因对编码。据推测，该位点是由重复引起的，随后出现序列分歧。我们比较了两对相邻基因的DNA序列，试图了解多样性产生和/或消除的方式。在整个转录区和侧翼区的重复序列之间发现了高水平的序列同一性（91%-99%），有两个显著的例外。首先，在编码绒毛膜蛋白的保守区域的DNA片段中，在39个碱基对的区域中检测到10个取代。这种局部区域的高变异性表明一个基因间转换样事件。第二，在编码蛋白质的羧基末端臂的区域中检测到141个碱基对的长度差异。这种差异可以通过由重复或三重事件在时间上分开的单个密码子（3个碱基对）的三个单独的重复来解释。
Two families of high-cysteine chorion proteins inBombyx moriare encoded in 15 tandemly arranged nonidentical gene pairs. It is assumed that this locus arose by duplication with subsequent sequence divergence. We have compared DNA sequences from two such neighboring pairs of genes in an attempt to understand the manner in which diversity has been generated and/or removed. A high level of sequence identity (91%–99%) was found between the repeats throughout the transcribed and flanking regions, with two significant exceptions. First, in the DNA segment encoding a conserved region of the chorion proteins, ten substitutions were detected in a 39-base-pair region. This localized region of high variability would suggest an intergene conversion-like event. Second, a length difference of 141 base pairs was detected in a region encoding the carboxy-terminal arm of the protein. This difference can be explained by three separate reiterations of single codons (3 base pairs) separated in time by duplication or triplication events.