Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast:: The importance of Mn2+ homeostasis
Pmr1, a P-type ATPase, and Pdt1, an Nramp homologue, cooperatively regulate cell morphogenesis in fission yeast:: The importance of Mn2+ homeostasis
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DOI：
10.1111/j.1356-9597.2004.00699.x
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发表时间：
2004-01-01
期刊：
影响因子：
2.1
通讯作者：
Kuno, T
中科院分区：
文献类型：
--
作者：
Maeda, T;Sugiura, R;Kuno, T
粟酒裂殖酵母 pmr1(+) 基因与酿酒酵母 PMR1 基因同源，编码 P 型 Ca2+/Mn2+ -ATP 酶。向培养基中添加 Mn2+ 以及 Ca2+ 会以钙调神经磷酸酶依赖性方式诱导 pmr1(+) 基因表达。 pmr1 敲除 (Deltapmr1) 细胞对 EGTA 表现出超敏反应。对 Deltapmr1 EGTA 超敏表型的高基因剂量抑制剂的筛选导致了 pdt1(+) 基因的鉴定，该基因编码 Nramp 相关金属转运蛋白。 Deltapmr1 细胞呈圆形细胞形态。尽管 Deltapdt1 细胞在常规培养基中表现正常，但当从培养基中除去 Mn2+ 时，它显示出与 Deltapmr1 细胞相似的圆形细胞形态。 Mn2+ 的去除也加剧了 Deltapmr1 细胞的圆形形态。 Deltapmr1Deltapdt1双突变体生长非常缓慢，并且表现出极其异常的细胞形态，呈圆形、增大和去极化形状。向培养基中添加Mn2+而非Ca2+完全抑制了形态缺陷，而Mn2+和Ca2+均显着改善了双突变体的缓慢生长。这些结果表明，Pmr1 和 Pdt1 通过控制 Mn2+ 稳态来协同调节细胞形态发生，并且钙调神经磷酸酶充当 Mn2+ 传感器和 Mn2+ 稳态调节剂。
Schizosaccharomyces pombe pmr1(+) gene is homologous to Saccharomyces cerevisiae PMR1 gene, which encodes the P-type Ca2+/Mn2+-ATPase. Addition of Mn2+, as well as Ca2+, to the medium induced pmr1(+) gene expression in a calcineurin-dependent manner. The pmr1 knockout (Deltapmr1) cells exhibited hypersensitivity to EGTA. A screen for high gene dosage-suppressors of the EGTA-hypersensitive phenotype of Deltapmr1 led to the identification of pdt1(+) gene, which encodes an Nramp-related metal transporter. The Deltapmr1 cells showed round cell morphology. Although Deltapdt1 cells appeared normal in the regular medium, it showed round cell morphology similar to that of the Deltapmr1 cells when Mn2+ was removed from the medium. The removal of Mn2+ also exacerbated the round morphology of the Deltapmr1 cells. The Deltapmr1Deltapdt1 double mutants grew very slowly and showed extremely aberrant cell morphology with round, enlarged and depolarized shape. The addition of Mn2+, but not Ca2+, to the medium completely suppressed the morphological defects, while both Mn2+ and Ca2+ markedly improved the slow growth of the double mutants. These results suggest that Pmr1 and Pdt1 cooperatively regulate cell morphogenesis through the control of Mn2+ homeostasis, and that calcineurin functions as a Mn2+ sensor as well as a Mn2+ homeostasis regulator.