Sodium taurocholate modifies the bile acid-independent fraction of canalicular bile flow in the rhesus monkey.
Sodium taurocholate modifies the bile acid-independent fraction of canalicular bile flow in the rhesus monkey.
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牛磺胆酸钠改变了恒河猴中胆小管胆汁流量的不依赖于胆汁酸的部分。
DOI：
10.1172/jci109453
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
J. Boyer
中科院分区：
文献类型：
--
作者：
A. Baker;R. Wood;A. Moossa;J. Boyer
在恒河猴胆总管内留置硅橡胶套管，测定胆汁酸非依赖性分泌和对牛磺胆酸盐的利胆反应。以3.5、7.0或10.5 mumol/min的随机顺序静脉输注胆汁酸，每次1.5 h。当用单回归线分析数据时，胆汁流量与胆汁酸分泌水平成比例增加，尽管y截距（胆汁酸非依赖性分泌的常规测量）变化很大（77.9+/-40.9 ml/24 h）。在动物之间和同一动物的重复研究中观察到y轴截距的变化，并且无法通过性别差异或留置硅橡胶套管的影响来解释，但似乎与胆汁酸输注顺序相关。只有两种牛磺胆酸输注速率（7.0和3.5 mumol/min）时，当初始胆汁酸输注处于高水平时，每摩尔分泌胆汁酸的[（14）C]清除率更高，但在低胆汁酸分泌速率时接近于零，这表明所谓的胆汁酸非依赖性小管流量与胆汁酸分泌密切相关或尺寸较小。当首先给予高输注速率时，[14] C清除率的增加也与[3 H]菊粉的胆汁清除率的增加有关，这表明菊粉的渗透性也增加了。用等摩尔的非胶束形成胆汁酸（牛磺去氢胆酸盐）代替牛磺胆酸盐的相同实验未能证明胆汁酸输注顺序对[3 H]菊粉的利胆反应或胆汁清除率有任何差异。这些实验表明，胶束形成胆汁酸，牛磺胆酸盐，可以增加大分子量溶质的胆汁系统的渗透性，并同时修改y轴截距和响应于转运的胆汁酸分泌的胆汁的体积。因此，牛磺胆酸盐可能通过改变胆汁分泌膜的结构或功能来改变其自身的利胆反应，并且似乎是恒河猴中所谓的胆汁酸非依赖性流动的主要决定因素。
Bile acid-independent secretion and the choleretic response to taurocholate were determined in rhesus monkeys fitted with indwelling silastic cannulas in the common bile ducts. Bile acids were infused intravenously in random order at 3.5, 7.0, or 10.5 mumol/min for 1.5 h each. When data were analyzed with a single regression line, bile flow increased in proportion to the level of bile acid secretion, although the y-intercepts (the conventional measurement of bile acid-independent secretion) varied widely (77.9+/-40.9 ml/24 h). The variation in y-intercepts was observed between animals and with repeated studies in the same animal and could not be explained by sex differences or the effects of the indwelling silastic cannulas, but seemed to be related to the order of bile acid infusion. With only two taurocholic acid infusion rates (7.0 and 3.5 mumol/min), [(14)C]erythritol clearance was greater per mole of secreted bile acid when the initial bile acid infusion was at the high level, but approached zero at low bile acid secretion rates, which suggests that so-called bile acid-independent canalicular flow is closely related to bile acid secretion or is small in size. The augmentation in [(14)C]erythritol clearance when the high infusion rate was given first was also associated with an increase in biliary clearance of [(3)H]inulin, which indicates that the premeability to inulin was also enhanced. Identical experiments which substituted equimolar infusions of a nonmicelle-forming bile acid (taurodehydrocholate) for taurocholate failed to demonstrate any difference in choleretic response or biliary clearance of [(3)H]inulin with the order of bile acid infusion. These experiments demonstrate that a micelleforming bile acid, taurocholate, can increase the permeability of the biliary system to large molecular weight solutes and simultaneously modify the y-intercept and the volume of bile secreted in response to the transported bile acid. Taurocholate may, therefore, modify its own choleretic response, perhaps by altering the structure or function of bile secretory membranes, and appears to be a major determinant of so-called bile acid-independent flow in rhesus monkeys.