Screening Method for Salmonella enterica Serovar Typhi and Serovar Paratyphi A with Reduced Susceptibility to Fluoroquinolones by PCR‐Restriction Fragment Length Polymorphism
Screening Method for Salmonella enterica Serovar Typhi and Serovar Paratyphi A with Reduced Susceptibility to Fluoroquinolones by PCR‐Restriction Fragment Length Polymorphism
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PCR-限制性片段长度多态性筛选对氟喹诺酮类药物敏感性降低的伤寒血清型和甲型副伤寒型血清型沙门氏菌
DOI：
10.1111/j.1348-0421.2003.tb02800.x
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发表时间：
2003
影响因子：
2.6
通讯作者：
Haruo Watanabe
中科院分区：
文献类型：
--
作者：
K. Hirose;K. Tamura;Haruo Watanabe
伤寒沙门氏菌和甲型副伤寒沙门氏菌对氟喹诺酮类药物(环丙沙星的最低抑菌浓度为0.25~2μg/ml)敏感性降低，在gyrA基因的Ser83或Asp-87密码子上存在突变。为筛选伤寒和甲型副伤寒临床分离株gyrA基因Ser-83和Asp-87密码子突变，设计了一种聚合酶链式反应-限制性片段长度多态(PCR-RFLP)筛查方法。该方法成功地筛查了对氟喹诺酮类药物敏感性降低的伤寒沙门氏菌和甲型副伤寒沙门氏菌的gyrA突变。
Salmonella enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones (MICs of ciprofloxacin, 0.25 to 2 μg/ml) have a mutation at codon either Ser‐83 or Asp‐87 of gyrA gene. A screening method by PCR‐restriction fragment length polymorphism (PCR‐RFLP) was designed to screen the mutations at codon Ser‐83 and Asp‐87 of the gyrA gene of S. enterica serovar Typhi and serovar Paratyphi A clinical isolates. This method successfully screened the gyrA mutations of S. enterica serovar Typhi and serovar Paratyphi A with reduced susceptibility to fluoroquinolones.