Short- and Long-Term Effects of UVA on Arabidopsis Are Mediated by a Novel cGMP Phosphodiesterase
Short- and Long-Term Effects of UVA on Arabidopsis Are Mediated by a Novel cGMP Phosphodiesterase
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DOI：
10.1016/j.cub.2019.06.071
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发表时间：
2019-08-05
期刊：
影响因子：
9.2
通讯作者：
Hetherington, Alistair M.
中科院分区：
文献类型：
--
作者：
Isner, Jean-Charles;Olteanu, Vlad-Aris;Hetherington, Alistair M.
虽然UVA辐射（315-400 nm）占到达地球表面的紫外线辐射的95%，但令人惊讶的是，人们对它对植物的影响知之甚少[1]。我们发现，在拟南芥中，短期暴露于UVA抑制气孔的开放，这需要降低胞质cGMP水平。这一过程不依赖于UVB受体UVR 8。cGMP激活的磷酸二酯酶（AtCN-PDE 1）是UVA诱导拟南芥cGMP减少的主要原因。AtCN-PDE 1样蛋白在大的HD结构域/PDEase样蛋白超家族中形成一个进化枝，但该亚家族的真核成员的功能尚未得到表征。这些基因已经从后生动物的基因组中丢失，但在其他方面作为单拷贝基因在生命树中保守。在长期实验中，UVA辐射促进了生长，降低了水的利用效率。这些实验表明，PDE 1也是生长的负调节剂。由于PDE 1基因是古老的，并且在动物谱系中没有代表，因此植物中的cGMP信号传导的至少一个元件可能与后生动物中存在的系统不同地进化。
Although UVA radiation (315-400 nm) represents 95% of the UV radiation reaching the earth's surface, surprisingly little is known about its effects on plants [1]. We show that in Arabidopsis, short- term exposure to UVA inhibits the opening of stomata, and this requires a reduction in the cytosolic level of cGMP. This process is independent of UVR8, the UVB receptor. A cGMP-activated phosphodiesterase (AtCN-PDE1) was responsible for the UVA-induced decrease in cGMP in Arabidopsis. AtCN-PDE1-like proteins form a clade within the large HD-domain/PDEase-like protein superfamily, but no eukaryotic members of this subfamily have been functionally characterized. These genes have been lost from the genomes of metazoans but are otherwise conserved as single-copy genes across the tree of life. In longer-term experiments, UVA radiation increased growth and decreased water-use efficiency. These experiments revealed that PDE1 is also a negative regulator of growth. As the PDE1 gene is ancient and not represented in animal lineages, it is likely that at least one element of cGMP signaling in plants has evolved differently to the system present in metazoans.