Zoonotic Ljungan Virus Associated with Central Nervous System Malformations in Terminated Pregnancy
Zoonotic Ljungan Virus Associated with Central Nervous System Malformations in Terminated Pregnancy
复制标题
DOI：
10.1002/bdra.20568
复制
发表时间：
2009-06-01
影响因子：
--
通讯作者：
Klitz, William
中科院分区：
文献类型：
--
作者：
Niklasson, Bo;Samsioe, Annika;Klitz, William
背景：在实验室小鼠模型中，已证实Ljungan病毒（LV）可导致中枢神经系统畸形。LV也与人类宫内胎儿死亡有关。我们调查了一系列选择性流产的脑积水和无脑畸形病例中左心室的存在。方法：采用免疫组化和真实的时间RT PCR检测了一系列因脑积水、无脑畸形和年龄相似的21三体选择性流产患者的LV。第二个实验涉及暴露于LV的新生小鼠。结果：10例脑积水中9例经免疫组化诊断为LV，18例21三体对照中1例经免疫组化诊断为LV。9例无脑畸形中有5例PCR结果阳性，而12例21三体可用于PCR检测的患者中无一例PCR结果阳性。47只暴露于LV的新生小鼠均出现脑炎，其中8只出现脑积水。52只对照动物均未出现脑炎或脑积水。结论：LV与脑积水和无脑畸形之间的关联表明LV可能在人类中枢神经系统畸形中起重要作用。出生缺陷研究（A部分）85：542-545，2009，（C）2009 Wiley-Liss，Inc.
BACKGROUND: The Ljungan virus (LV) has been shown to cause central nervous system malformations in laboratory mouse models. The LV has also been associated with intrauterine fetal death in humans. We investigated the presence of LV in a series of human hydrocephaly and anencephaly cases from elective abortions. METHODS: A series of elective abortions owing to hydrocephaly, anencephaly, and similarly aged trisomy 21 elective abortions as controls were examined for LV by immunohistochemistry and real time RTPCR. A second experiment involved newborn mice exposed to LV. RESULTS: LV was diagnosed in 9 of 10 cases with hydrocephalus and in I of 18 trisomy 21 controls by immunohistochemistry. Five of nine cases with anencephaly had a positive PCR result, whereas none of the 12 trisomy 21 available for PCR testing had a positive result. The 47 newborn mice exposed to LV all developed encephalitis, with eight having hydrocephalus. None of the 52 control animals had encephalitis or hydrocephalus. CONCLUSION: The association between LV and both hydrocephaly and anencephaly suggests that LV may be playing an important role in central nervous system malformations in humans. Birth Defects Research (Part A) 85:542-545, 2009, (C) 2009 Wiley-Liss, Inc.