Development of a thermogelling ophthalmic formulation of cysteine
Development of a thermogelling ophthalmic formulation of cysteine
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DOI：
10.1080/03639040500390934
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发表时间：
2006-01-01
影响因子：
3.4
通讯作者：
Chaumeil, JC
中科院分区：
文献类型：
--
作者：
Dumortier, G;El Kateb, N;Chaumeil, JC
用半胱氨酸2%溶液进行的初步研究表明，将pH调节到等电pH(即4.9)可增强高压灭菌过程中的稳定性，并确保如果在2-8摄氏度的避光条件下储存，至少14天内不会显著降解。优化的配方将半胱氨酸(2%)/泊洛沙姆407(16.5%)或cysteine(2%)/Poloxamer407(20%)/Poloxamer188(5%)结合在一起，具有适当的凝胶温度(Tg)(分别为25.9℃和26.9℃)、重要的凝胶强度(分别为5.1daN和5.3daN)以及在24℃和32℃之间的表观粘度急剧增加(增殖倍数分别为78倍和77倍)。添加半胱氨酸只会轻微但显著地降低凝胶温度和提高凝胶强度。
Preliminary studies carried out with cysteine 2% solution showed that pH adjusted to isoelectrical pH (i.e., 4.9) led to enhance stability during autoclaving and ensured no significant degradation during at least 14 days if stored at 2-8 degrees C protected from light. Optimized formulations combined either cysteine(2%)/Poloxamer407(16.5%) or cysteine(2%)/Poloxamer407(20%)/Poloxamer188(5%) and were characterized by an adequate temperature of gelification (TG) (25.9 degrees C and 26.9 degrees C, respectively), an important gel strength (5.1daN and 5.3daN, respectively) and a drastic increase in the apparent viscosity between 24 degrees C and 32 degrees C (multiplication factor of 78 and 77-fold, respectively). Cysteine addition produced only slight but significant decrease in temperature of gelification and increase in gel strength.