H2A.Z is required for global chromatin integrity and for recruitment of RNA polymerase II under specific conditions
H2A.Z is required for global chromatin integrity and for recruitment of RNA polymerase II under specific conditions
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DOI：
10.1128/mcb.21.18.6270-6279.2001
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发表时间：
2001-09-01
影响因子：
5.3
通讯作者：
Gaudreau, L
中科院分区：
文献类型：
--
作者：
Adam, M;Robert, F;Gaudreau, L
进化上保守的组蛋白变异体H_2A.Z最近被证明在酿酒酵母中调节基因转录。在这里，我们表明，在这个生物体中失去H2A.Z对GAL基因的诱导产生负面影响。重要的是，将H_2A.Z的C-末端区域与S相H_2A融合，而不是其相应的C-末端区域，可以介导组蛋白变异体在GAL1-10基因诱导中的特异性功能，并通过HTZ1的缺失恢复细胞的缓慢生长表型。此外，我们还发现R2A.Z的C-末端区域可以与转录装置的一些组件相互作用。在缺乏H2A.Z的细胞中，在诱导条件下，RNA聚合酶II和TATA结合蛋白对GAL1-10启动子的募集显著减少。出乎意料的是，我们还发现在GAL基因诱导的条件下，H2A.Z需要在全球范围内保持染色质的完整性。我们假设，在特定的细胞生长条件下，H2A.Z至少部分地通过调节特定基因与RNA聚合酶II相关因子的相互作用来正向调节基因转录。
Evolutionarily conserved variant histone H2A.Z has been recently shown to regulate gene transcription in Saccharomyces cereviside. Here we show that loss of H2A.Z in this organism negatively affects the induction of GAL genes. Importantly, fusion of the H2A.Z C-terminal region to S phase H2A without its corresponding C-terminal region can mediate the variant histone's specialized function in GAL1-10 gene induction, and it restores the slow-growth phenotype of cells with a deletion of HTZ1. Furthermore, we show that the C-terminal region of R2A.Z can interact with some components of the transcriptional apparatus. In cells lacking H2A.Z, recruitment of RNA polymerase II and TATA-binding protein to the GAL1-10 promoters is significantly diminished under inducing conditions. Unexpectedly, we also find that H2A.Z is required to globally maintain chromatin integrity under GAL gene-inducing conditions. We hypothesize that H2A.Z can positively regulate gene transcription, at least in part, by modulating interactions with RNA polymerase II-associated factors at certain genes under specific cell growth conditions.