Murine CD1d-restricted T cell recognition of cellular lipids
Murine CD1d-restricted T cell recognition of cellular lipids
复制标题
DOI：
10.1016/s1074-7613(00)80174-0
复制
发表时间：
2000-02-01
期刊：
影响因子：
32.4
通讯作者：
Behar, SM
中科院分区：
文献类型：
--
作者：
Gumperz, JE;Roy, C;Behar, SM
NKT细胞与自身免疫性疾病和癌症的免疫控制有关，并且可以在不添加外源性抗原的情况下识别细胞表面mCD1d。mCD1d呈递的细胞抗原尚未被鉴定，尽管NKT细胞可以识别合成糖脂，α - galcer。在这里，我们展示了在加入肿瘤细胞系的脂质提取物后，平板结合的mCD1d分子刺激了NKT细胞杂交瘤。该杂交瘤也对三种纯化磷脂有强烈反应，但不能识别α - galcer。其他16个mCD1d限制性杂交瘤中有7个也对某些纯化磷脂有反应。这些发现表明NKT细胞可以识别不同于α - galcer的细胞抗原，并识别磷脂作为mCD1d呈递的潜在自身抗原。
NKT cells are associated with immunological control of autoimmune disease and cancer and can recognize cell surface mCD1d without addition of exogenous antigens. Cellular antigens presented by mCD1d have not been identified, although NKT cells can recognize a synthetic glycolipid, alpha-GalCer. Here we show that after addition of a lipid extract from a tumor cell line, plate-bound mCD1d molecules stimulated an NKT cell hybridoma. This hybridoma also responded strongly to three purified phospholipids, but failed to recognize alpha-GalCer. Seven of sixteen other mCD1d restricted hybridomas also showed a response to certain purified phospholipids. These findings suggest NKT cells can recognize cellular antigens distinct from alpha-GalCer and identify phospholipids as potential self-antigens presented by mCD1d.