Differentiation patterns in two- and three-dimensional culture systems of human squamous carcinoma cell lines.
Differentiation patterns in two- and three-dimensional culture systems of human squamous carcinoma cell lines.
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发表时间：
1990-09
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通讯作者：
Ruth Knuechel;Peter C. Keng;Ferdinand Hofstaedter;V. Langmuir;Robert M. Sutherland;David P. Penney
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作者：
Ruth Knuechel;Peter C. Keng;Ferdinand Hofstaedter;V. Langmuir;Robert M. Sutherland;David P. Penney
对A431和CaSki两种女性生殖道鳞状细胞癌细胞系在不同实验组织培养状态下的分化模式进行了相对定量的研究，这两种实验组织培养状态经常用于评估药物和辐射对人类肿瘤的影响。比较了单层和多细胞肿瘤球体(MCTS)的二维和三维体外培养，以及作为体内肿瘤模型的裸鼠-异种移植瘤。此外，在体外实验中比较使用了表皮生长因子(EGF)。从单层到MCTS再到异种移植瘤，两种细胞系均可识别出上皮细胞分化的形态标志。细胞角蛋白(CK)在A431细胞中稳定表达。然而，利用免疫组织化学方法，当将抗体8.12应用于单层细胞时，发现CK抗原的部分掩蔽，并可通过流式细胞仪测量来定量。在CaSki异种移植瘤中发现了基本的细胞变化，在肿瘤生长后期表现出新的角化性癌的特征。表皮生长因子导致MCTS细胞间接触减少和较晚发生坏死，导致球体的存活率增加。肿瘤模型系统分化的显著差异表明，免疫组织化学和流式细胞术的分化特征对于帮助解释这些不同的肿瘤模型所获得的数据是必要的。
Relative quantification of the pattern of differentiation of two squamous carcinoma cell lines of the female genital tract, A431 and CaSki, was studied in various experimental tissue culture states that are frequently used to evaluate drug and radiation effects on human tumors. Two- and three-dimensional in vitro cultures, ie, monolayers and multicellular tumor spheroids (MCTS), and nude mice-xenograft tumors as in vivo tumor models were compared. In addition, epidermal growth factor (EGF) was used comparatively in the in vitro studies. Morphologic signs of epithelial differentiation could be recognized in both cell lines gradually increasing from monolayers to MCTS to xenograft tumors. Cytokeratin (CK) expression is described as stable in A431 cells. Using immunohistochemistry, however, partial masking of CK antigens was found when applying the antibody 8.12 on monolayer cells and could be quantified by flow cytometric measurements. Fundamental cellular changes were found in a CaSki xenograft tumor, which showed newly established features of a keratinizing carcinoma after late onset of tumor growth. Epidermal growth factor caused reduction of both intercellular contacts and later onset of necrosis in MCTS, leading to an increased viability of the spheroids. Significant differences in differentiation of the tumor model systems indicates that the characterization of differentiation with immunohistochemistry and flow cytometry is necessary to assist interpretation of data obtained with these different tumor models.