Transcriptome Analysis of Early Organogenesis in Human Embryos
Transcriptome Analysis of Early Organogenesis in Human Embryos
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人类胚胎早期器官发生的转录组分析
DOI：
10.1016/j.devcel.2010.06.014
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发表时间：
2010-07-20
影响因子：
11.8
通讯作者：
Jin, Ying
中科院分区：
文献类型：
--
作者：
Fang, Hai;Yang, Ying;Jin, Ying
胚胎发育的全基因组表达分析提供了在各种情况下有用的信息。在这里，我们报告了人类早期胚胎的转录组谱，涵盖了器官发生的前三分之一的发育过程。我们确定了两大类基因，在这个发育窗口中表现出逐渐减少或逐渐增加的表达模式。减少组似乎包括对器官发生起始重要的干性特异性和分化特异性基因，而增加组似乎主要与分化相关并指示不同的器官形成。基于这些发现，我们设计了一个假定的分子网络，可以为早期人类器官发生的调节提供框架。我们的结果代表了早期人类胚胎发生特征的重要一步，并为理解人类发育和干细胞工程提供了资源。
Genome-wide expression analysis of embryonic development provides information that is useful in a variety of contexts. Here, we report transcriptome profiles of human early embryos covering development during the first third of organogenesis. We identified two major categories of genes, displaying gradually reduced or gradually increased expression patterns across this developmental window. The decreasing group appeared to include stemness-specific and differentiation-specific genes important for the initiation of organogenesis, whereas the increasing group appeared to be largely differentiation related and indicative of diverse organ formation. Based on these findings, we devised a putative molecular network that may provide a framework for the regulation of early human organogenesis. Our results represent a significant step in characterization of early human embryogenesis and provide a resource for understanding human development and for stem cell engineering.