High expression of stratifin is a universal abnormality during the course of malignant progression of early-stage lung adenocarcinoma
High expression of stratifin is a universal abnormality during the course of malignant progression of early-stage lung adenocarcinoma
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DOI：
10.1002/ijc.25907
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发表时间：
2011-11-15
影响因子：
6.4
通讯作者：
Noguchi, Masayuki
中科院分区：
文献类型：
--
作者：
Shiba-Ishii, Aya;Kano, Junko;Noguchi, Masayuki
肺原位腺癌（AIS）预后极好，5年生存率为100%。然而，早期浸润性腺癌（EIA）通常会导致致命的后果。在本研究中，我们比较了AIS与EIA显示淋巴结转移或致命结果的表达谱，并通过cDNA微阵列筛选差异表达基因。从选择的基因中，我们重点关注 Stratifin（SFN，14-3-3 sigma），它在 EIA 中的表达显着高于 AIS 中。 SFN 的免疫组织化学显示，超过 95% 的 EIA 呈 SFN 免疫阳性，而 AIS 中只有 13% (p < 0.05)。有趣的是，不仅在侵入区域，而且在 EIA 的原位传播部分也检测到了阳性。从功能上讲，SFN 促进肺腺癌的细胞增殖能力。这些结果表明，SFN 过度表达是肺原位腺癌逐步进展过程中的普遍异常。
Adenocarcinoma in situ (AIS) of the lung has an extremely favorable prognosis, with a 5-year survival rate of 100%. However, early invasive adenocarcinoma (EIA) often has a fatal outcome. In this study, we compared the expression profiles of AIS with those of EIA showing lymph node metastasis or a fatal outcome, and screened the differentially expressed genes by cDNA microarray. From the genes selected, we focused on Stratifin (SFN, 14-3-3 sigma), which showed significantly higher expression in EIA than in AIS. Immunohistochemistry for SFN revealed that more than 95% of EIAs were immunopositive for SFN, in comparison to only 13% of AISs (p < 0.05). Interestingly, positivity was detected not only in the invasive region but also in the in situ spreading component of EIA. Functionally, SFN facilitates the cell proliferation capacity of lung adenocarcinoma. These results indicate that SFN overexpression is a universal abnormality during the stepwise progression from in situ to invasive adenocarcinoma of the lung.