Differential and stage-related expression in embryonic tissues of a new human homoeobox gene
Differential and stage-related expression in embryonic tissues of a new human homoeobox gene
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DOI：
10.1038/324664a0
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发表时间：
1986-12
期刊：
影响因子：
64.8
通讯作者：
F. Mavilio;A. Simeone;A. Giampaolo;A. Faiella;V. Zappavigna;D. Acampora;G. Poiana;G. Russo;C. Peschle;E. Boncinelli
中科院分区：
文献类型：
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作者：
F. Mavilio;A. Simeone;A. Giampaolo;A. Faiella;V. Zappavigna;D. Acampora;G. Poiana;G. Russo;C. Peschle;E. Boncinelli
同源盒是一个183个碱基对(Bp)的DNA序列，在几种果蝇中保守，控制节段和节段识别2-4。从昆虫和鞍类动物到脊椎动物5，6的基因组中都发现了同源盒序列，从爪哇7-9，老鼠10-22和man23克隆了包含同源盒的基因。我们最近分离了包含互补DNA克隆的人类同源盒，这些克隆代表了四个不同人类基因的转录本24。一个克隆(HHO.c10)在受精后5-10周的人类胚胎和胎儿的脊髓中选择性地表达在2.1kb的多腺苷酸化转录本中。我们报告了第二个cdna克隆，命名为HHO.c13，它代表了一个新的同源框基因。该克隆编码一个255个氨基酸残基的蛋白质，其中包括一个位于Homeoo结构域上游的五肽，在其他果蝇、非洲爪哇、小鼠和人类Homeobox基因中保守。通过Northern分析，HHO.c13检测到多个胚胎转录本，这些转录本在5-9周大的人类胚胎和胎儿的脊髓、脑、脊柱、四肢芽和心脏中差异表达，具有显著的器官和阶段特异性模式。这些观察表明，在哺乳动物早期发育中，同源框基因可能在除脊髓以外的各种器官和身体部位发挥广泛的控制功能。
The homoeobox is a 183 base-pair (bp) DNA sequence1conserved in severalDrosophilagenes controlling segmentation and segment identity2–4. Homoeobox sequences have been detected in the genome of species ranging from insects and anellids to vertebrates5,6and homoeobox containing genes have been cloned fromXenopus7–9, mouse10–22and man23. We recently isolated human homoeobox containing complementary DNA clones, that represent transcripts from four different human genes24. One clone (HHO.c10) is selectively expressed in a 2.1 kilobase (kb) polyadenylated transcript in the spinal cord of human embryos and fetuses 5–10 weeks after fertilization25. We report the characterization of a second cDNA clone, termed HHO.c13, that represents a new homoeobox gene. This clone encodes a protein of 255 amino-acid residues, which includes a pentapeptide, upstream of the homoeo domain, conserved in otherDrosophila, Xenopus, murine and human homoeobox genes. By Northern analysis HHO.c13 detects multiple embryonic transcripts, which are differentially expressed in spinal cord, brain, backbone rudiments, limb buds and heart in 5–9-week-old human embryos and fetuses, in a striking organ- and stage-specific pattern. These observations suggest that in early mammalian development homoeobox genes may exert a wide spectrum of control functions in a variety of organs and body parts, in addition to the spinal cord.