Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins.
Functionalized terahertz plasmonic metasensors: Femtomolar-level detection of SARS-CoV-2 spike proteins.
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DOI：
10.1016/j.bios.2021.112971
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发表时间：
2021-04-01
影响因子：
12.6
通讯作者：
Ghoreishi SA
中科院分区：
文献类型：
--
作者：
Ahmadivand A;Gerislioglu B;Ramezani Z;Kaushik A;Manickam P;Ghoreishi SA
有效和高效地管理人类贝塔冠状病毒严重急性呼吸系统综合症-冠状病毒2型病毒感染，即新冠肺炎大流行，需要具有较短样本到结果持续时间的敏感和选择性传感器来执行所需的诊断。在这个方向上，一种合适的低水平检测SARS-CoV-2病毒蛋白的替代方法，即毫微米(FM)正在探索用于新冠肺炎诊断的等离子体元传感器技术，这为高级医疗保健计划和现代临床诊断提供了极好的机会。等离子体元传感器的固有优点源于其在频率、时间和空间上同时压缩电磁场的能力。然而，在低密度下检测低分子生物分子是传统超传感器的一个典型缺陷，最近使用环状超表面技术解决了这一问题。本研究致力于制作一种基于环形电动力学概念的小型化等离子体免疫传感器，该传感器可以在太赫兹(THz)频率下支持具有超箭头线形的强健受限等离子体模式。通过使用我们的准无限亚表面激发环偶极子模式，以及基于官能化的金纳米颗粒(AuNPs)与SARS-CoV-2病毒S蛋白(S1)的特异性单抗偶联的明智优化的方案，我们可以监测不同浓度的S蛋白的共振位移。相对分子质量在76 kDa左右时，检测SARS-CoV-2病毒蛋白的最低检出限(LOD)为4.2 kDa。我们预计，这项研究的结果将为使用环形元传感器作为实用技术铺平道路，用于在医院、诊所、实验室和感染地点快速和准确地筛查SARS-CoV-2病毒携带者、有症状或无症状的病毒携带者和刺突蛋白。
Effective and efficient management of human betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 virus infection i.e., COVID-19 pandemic, required sensitive and selective sensors with short sample-to-result durations for performing desired diagnostics. In this direction, one appropriate alternative approach to detect SARS-CoV-2 virus protein at low level i.e., femtomolar (fM) is exploring plasmonic metasensor technology for COVID-19 diagnostics, which offers exquisite opportunities in advanced healthcare programs, and modern clinical diagnostics. The intrinsic merits of plasmonic metasensors stem from their capability to squeeze electromagnetic fields, simultaneously in frequency, time, and space. However, the detection of low-molecular weight biomolecules at low densities is a typical drawback of conventional metasensors that has recently been addressed using toroidal metasurface technology. This research is focused on the fabrication of a miniaturized plasmonic immunosensor based on toroidal electrodynamics concept that can sustain robustly confined plasmonic modes with ultranarrow lineshapes in the terahertz (THz) frequencies. By exciting toroidal dipole mode using our quasi-infinite metasurface and a judiciously optimized protocol based on functionalized gold nanoparticles (AuNPs) conjugated with the specific monoclonal antibody specific to spike protein (S1) of SARS-CoV-2 virus onto the metasurface, the resonance shifts for diverse concentrations of the spike protein are monitored. Possessing molecular weight around ~76 kDa allowed to detect the presence of SARS-CoV-2 virus protein with significantly low as limit of detection (LoD) was achieved as ~4.2 fM. We envisage that outcomes of this research will pave the way toward the use of toroidal metasensors as practical technologies for rapid and precise screening of SARS‐CoV‐2 virus carriers, symptomatic or asymptomatic, and spike proteins in hospitals, clinics, laboratories, and site of infection.
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