Patterning and regeneration of surfaces with antibodies.
Patterning and regeneration of surfaces with antibodies.
复制标题
用抗体对表面进行图案化和再生。
DOI：
10.1021/ac00115a034
复制
发表时间：
1995
影响因子：
7.4
通讯作者：
J. Cooper
中科院分区：
文献类型：
--
作者：
D. Pritchard;H. Morgan;J. Cooper
我们描述了一种新技术的应用，用于在200微米x 50微米的区域内固定六种不同的蛋白质。这个过程包括在表面涂上去糖基化的亲和素，它与光活性生物素类似物结合，因此提供了一个可光定位的表面，蛋白质可以固定在上面。这是第一次描述了许多不同蛋白质的连续模式，我们通过制造一个免疫点来证明这一点，以便对多种抗原的结合进行定性可视化。我们还表明，这种结构表面可以很容易地再生并随后重新图案。
We describe the application of a new technique for the site-specific immobilization of six different proteins within an area of 200 microns x 50 microns. The procedure involves coating the surface with deglycosylated avidin, which binds a photoactive biotin analogue, so providing a light-addressable surface onto which proteins can be immobilized. This is the first time that consecutive patterning of a number of different proteins has been described, and we demonstrate this with the fabrication of an immunospot for the qualitative visualization of the binding of multiple antigens. We also show that such a structured surface can be readily regenerated and subsequently repatterned.