Distinct HDL subclasses present similar intrinsic susceptibility to oxidation by HOCl
Distinct HDL subclasses present similar intrinsic susceptibility to oxidation by HOCl
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DOI：
10.1016/j.abb.2009.05.005
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发表时间：
2009-07-01
影响因子：
3.9
通讯作者：
Kontush, Anatol
中科院分区：
文献类型：
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作者：
Chantepie, Sandrine;Malle, Ernst;Kontush, Anatol
血红素蛋白髓过氧化物酶(MPO)是脂蛋白氧化的催化剂。次氯酸(HOCl)是一种由活化的吞噬细胞的MPO-H(2)O(2)-Cl2体系形成的有效的双电子氧化剂，可修饰抗动脉粥样硬化的高密度脂蛋白(HDL)。用高密度脂蛋白亚组分的结构不均一性和氧化敏感性用高氯酸根进行了探讨。5种不同的高密度脂蛋白亚组分均被HOCl修饰，表现为色氨酸残基和游离氨基的消耗、载脂蛋白A1的交联化、HOCl修饰表位的形成、电泳迁移率的增加以及不饱和脂肪酸含量的改变。在HOCl：HDL2质量比固定为1：32的情况下，在总质量基础上，小而致密的HDL3比大而轻的HDL2更不容易被氧化修饰。但相反，在HOCl：HDL摩尔比为97：1的固定条件下，不是以颗粒数为基础的。我们得出的结论是，HDL亚类之间的结构和物理化学差异并不影响它们对HOCl氧化攻击的固有敏感性。(C)2009 Elsevier Inc.保留所有权利。
The heme protein myeloperoxidase (MPO) functions as a catalyst for lipoprotein oxidation. Hypochlorous acid (HOCl), a potent two-electron oxidant formed by the MPO-H(2)O(2)-chloride system of activated phagocytes, modifies antiatherogenic high-density lipoprotein (HDL). The structural heterogeneity and oxidative susceptibility of HDL particle subfractions were probed with HOCl. All distinct five HDL subfraction were modified by HOCl as demonstrated by the consumption of tryptophan residues and free amino groups, cross-linking of apolipoprotein Al, formation of HOCl-modified epitopes, increased electrophoretic mobility and altered content of unsaturated fatty acids in HDL Subclasses. Small, dense HDL3 were less susceptible to oxidative modification than large, light HDL2 on a total mass basis at a fixed HOCl:HDL mass ratio of 1:32. but in contrast not on a particle number basis at a fixed HOCl:HDL molar ratio of 97:1. We conclude that structural and physicochemical differences between HDL subclasses do not influence their intrinsic Susceptibility to oxidative attack by HOCl. (C) 2009 Elsevier Inc. All rights reserved.