Growth advantage and enhanced toxicity of Escherichia coli adherent to tissue culture cells due to restricted diffusion of products secreted by the cells.
Growth advantage and enhanced toxicity of Escherichia coli adherent to tissue culture cells due to restricted diffusion of products secreted by the cells.
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由于细胞分泌产物的扩散受到限制，大肠杆菌粘附在组织培养细胞上的生长优势和增强的毒性。
DOI：
10.1172/jci112939
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Ofek,I
中科院分区：
文献类型：
--
作者：
Zafriri,D;Oron,Y;Eisenstein,BI;Ofek,I
本研究旨在研究粘附于组织细胞的大肠杆菌是否由于其与细胞的接近而获得优于非粘附细菌的优势。我们使用组织培养细胞和脯氨酸营养缺陷型大肠杆菌的同基因衍生物。大肠杆菌的菌毛型（Fim+）和非菌毛型（Fim-），以及产热不稳定肠毒素型（Tox+）和不产毒素型（Tox-）。我们发现，Fim+细菌，这是能够粘附到组织培养细胞，启动生长快得多，比nonadhesive Fim-细菌;粘附细菌使用组织细胞来源的脯氨酸，这是在高浓度，只有在细菌粘附区。同样，粘附（Fim+）细菌分泌的环AMP保持在组织细胞表面上的高浓度。只有2 × 10（5）个粘附的Fim+ Tox+细菌对Y1肾上腺细胞产生毒性活性，而6 × 10（6）个Fim-Tox+细菌在培养基中分泌的毒素检测不到。结果表明，粘附性大肠杆菌的生长优势和增强的毒性。这是由于组织培养物和细菌细胞分泌的产物的扩散受到限制。图片
This study was undertaken to examine whether Escherichia coli adherent to tissue cells gain advantages over nonadherent bacteria due to their proximity to the cells. We used tissue culture cells and isogenic derivatives of a proline auxotrophic strain of E. coli that were fimbriated (Fim+) or nonfimbriated (Fim-), and were heat-labile enterotoxin producing (Tox+) or toxin nonproducing (Tox-). We found that the Fim+ bacteria; which were capable of adhering to tissue culture cells, initiated growth much sooner than did nonadherent Fim- bacteria; the adherent bacteria used tissue cell-derived proline, which was available at high concentrations only in the zone of bacterial adherence. Likewise, cyclic AMP secreted by adherent (Fim+) bacteria was maintained at high concentration on the tissue cell surfaces. As few as 2 X 10(5) adherent Fim+ Tox+ bacteria exert toxic activity upon Y1 adrenal cells, whereas toxin secreted in the medium by 6 X 10(6) Fim- Tox+ bacteria was undetectable. The results suggest that the growth advantage and enhanced toxicity of adherent E. coli is due to restricted diffusion of products secreted by the tissue culture and bacterial cells, respectively.Images