Spaceflight downregulates antioxidant defense systems in rat liver
Spaceflight downregulates antioxidant defense systems in rat liver
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DOI：
10.1016/s0891-5849(97)00278-5
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发表时间：
1998-01-15
影响因子：
7.4
通讯作者：
Ji, LL
中科院分区：
文献类型：
--
作者：
Hollander, J;Gore, M;Ji, LL
将雄性Sprague-Dawley大鼠置于航天飞机STS-63的封闭模块中8 d（F，n = 6），研究肝脏抗氧化酶活性、mRNA丰度和谷胱甘肽（GSH）状态。将F动物与饲养在地面封闭模块中的大鼠（G，n = 9）进行比较，模拟与发射和飞行相关的振动和温度条件，并将大鼠在常规地面饲养条件下饲养在单独的笼子中（V，n = 6）。航天飞行显著降低了肝脏中的过氧化氢酶、GSH还原酶和GSH硫转移酶活性（p <0.05）。铜锌锰超氧化物歧化酶（SOD）的酶活性和酶蛋白含量不受飞行的影响。与G组大鼠相比，F组大鼠Cu-Zn SOD和过氧化氢酶mRNA的相对丰度显著降低（p <0.05）。太空飞行导致肝脏GSH、谷胱甘肽二硫化物和总GSH含量显著降低（p <0.01），并伴有较低的γ-谷氨酰转肽酶活性（p <0.05）。与G和V大鼠相比，F大鼠的肝脏丙二醛浓度增加了47%（p <0.05）。肝脏蛋白质含量不受飞行的影响。这些结果表明，航天飞行可以下调抗氧化防御能力，并引起肝脏的氧化应激。(C)1998年爱思唯尔科学公司
Liver antioxidant enzyme activities, mRNA abundance, and glutathione (GSH) status were investigated in male Sprague-Dawley rats placed in an enclosure module aboard Space Shuttle STS-63 for 8 d (F, n = 6). F animals were compared to rats housed in an enclosure module on the ground (G, n = 9), which simulated the vibration and temperature conditions associated with launch and flight, and rats kept under conventional ground vivarium conditions in individual cages (V, n = 6). Spaceflight significantly decreased catalase, GSH reductase, and GSH sulfur-transferase activities in the liver (p < .05). Neither enzyme activity nor enzyme protein content of Cu-Zn and Mn superoxide dismutase (SOD) was affected by flight. The relative abundance of mRNA for Cu-Zn SOD and catalase was significantly decreased comparing F with G rats (p < .05). Spaceflight resulted in a dramatic decrease of liver GSH, glutathione disulfide, and total GSH contents (p < .01),which were accompanied by a lower gamma-glutamyl transpeptidase activity (p < .05). F rats showed a 47% (p < .05) increase in liver malondialdehyde concentration compared to G and V rats. Liver protein content was not affected by flight. These results indicate that spaceflight can downregulate antioxidant defense capacity and elicit an oxidative stress in the liver. (C) 1998 Elsevier Science Inc.