Reversed functional topology in the antennal lobe of the male European corn borer
Reversed functional topology in the antennal lobe of the male European corn borer
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DOI：
10.1242/jeb.017319
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发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Hansson, Bill S.
中科院分区：
文献类型：
--
作者：
Karpati, Zsolt;Dekker, Teun;Hansson, Bill S.
欧洲玉米螟Ostrinia nubilalis （Hubner）是昆虫性交流进化的典范。两种信息素菌株对两种信息素成分Z11和e11 -十四烯乙酸酯的产生和响应比例相反。z型菌株使用Z11:E11十四烯乙酸酯的比例为97:3，而e型菌株使用1:99的比例。我们研究了雄性偏好的差异如何与触角叶（AL）嗅觉输入和输出神经元的连接差异相关。利用活性依赖的顺行染色、细胞内记录和免疫细胞化学等方法建立雄性嗅觉受体神经元（orn）和AL投射神经元（PNs）的结构和功能。用α -突触蛋白染色的ALs共聚焦显微镜重建具有生理特征的神经元。两株雄性和雌性的ALs均约有64个肾小球。雄性大肾小球复合体（MGC）在两个菌株中形态相似，由两个主要的室组成，一个大的内侧室围绕着一个较小的外侧室折叠。广泛的生理和形态学分析表明，在这两种菌株中，主要信息素成分特异性的orn和PNs主要分布在MGC内侧肾小球，而对次要信息素成分敏感的orn和PNs主要分布在外侧肾小球。换句话说，这两个菌株具有不可区分的MGC形态，但拓扑结构相反。显然，导致行为根本改变的单基因介导的转移位于触角叶的上游，即在ORN水平。
The European corn borer Ostrinia nubilalis (Hubner) is a model of evolution of sexual communication in insects. Two pheromone strains produce and respond to opposite ratios of the two pheromone components, Z11 and E11-tetradecenylacetate. The Z-strain uses a ratio of 97:3 of Z11:E11 tetradecenylacetate, whereas the E-strain uses a ratio of 1:99. We studied how the difference in male preference correlates with differences in wiring of olfactory input and output neurons in the antennal lobe (AL). Activity-dependent anterograde staining, intracellular recording and immunocytochemistry were used to establish the structure and function of male olfactory receptor neurons (ORNs) and AL projection neurons (PNs). Physiologically characterized neurons were reconstructed using confocal microscopy of alpha-synapsin stained ALs. The ALs of males and females in both strains had approximately 64 glomeruli. In males the macroglomerular complex (MGC) was morphologically similar in the two strains and consisted of two major compartments, a large, medial compartment folded around a smaller, lateral one. Extensive physiological and morphological analysis revealed that in both strains the major pheromone component-specific ORNs and PNs arborize in the medial MGC glomerulus, whereas those sensitive to the minor pheromone component arborize in the lateral glomerulus. In other words, the two strains have an indistinguishable MGC morphology, but a reversed topology. Apparently, the single-gene-mediated shift that causes a radical change in behavior is located upstream of the antennal lobes, i.e. at the ORN level.