Molecular cloning of porcine growth differentiation factor 9 (GDF-9) cDNA and its role in early folliculogenesis: direct ovarian injection of GDF-9 gene fragments promotes early folliculogenesis.
Molecular cloning of porcine growth differentiation factor 9 (GDF-9) cDNA and its role in early folliculogenesis: direct ovarian injection of GDF-9 gene fragments promotes early folliculogenesis.
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DOI：
10.1530/rep.1.00224
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发表时间：
2004-11
期刊：
影响因子：
3.8
通讯作者：
Takashi Shimizu;Yasunori Miyahayashi;M. Yokoo;Y. Hoshino;H. Sasada;E. Sato
中科院分区：
文献类型：
--
作者：
Takashi Shimizu;Yasunori Miyahayashi;M. Yokoo;Y. Hoshino;H. Sasada;E. Sato
生长分化因子-9 (GDF-9) 是生长中的卵泡中的卵母细胞分泌的生长因子。为了研究GDF-9在猪中的卵巢功能，我们首先克隆了猪GDF-9互补DNA（cDNA），然后将其基因片段注射到后备母猪的卵巢中。猪 GDF-9 与人、牛、绵羊、大鼠和小鼠对应物的开放阅读框 (ORF) 同源性分别为 81.4%、84.6%、84.2%、72.7% 和 72.6%。推导的成熟蛋白的氨基酸序列，相应的同源性分别达到92.1%、97.8%、97.0%、89.6%和88.1%。为了研究 GDF-9 在早期卵泡发生中的作用，对 2 个月大的青春期前母猪的卵巢注射了 GDF-9 基因片段。注射猪GDF-9基因片段导致初级、次级和三级卵泡数量增加，同时原始卵泡数量减少。这些结果表明，外源性GDF-9可以促进猪卵巢的早期卵泡发生，直接卵巢注射GFD-9基因片段的技术可能有助于预防和治疗卵巢功能障碍相关不孕症的新疗法。
Growth differentiation factor-9 (GDF-9) is a growth factor secreted by oocytes in growing ovarian follicles. To investigate the ovarian function of GDF-9 in pigs, we first cloned porcine GDF-9 complementary DNA (cDNA), and then injected its gene fragments into the ovary in gilts. Porcine GDF-9 has open reading frame (ORF) homologies of 81.4%, 84.6%, 84.2%, 72.7% and 72.6% with its human, bovine, ovine, rat and mouse counterparts respectively. Regarding the deduced amino-acid sequence of the mature protein, the corresponding homologies reach 92.1%, 97.8%, 97.0%, 89.6% and 88.1% respectively. To investigate the role of GDF-9 in early folliculogenesis, the ovaries of 2-month-old prepubertal gilts were injected with GDF-9 gene fragments. The injection of porcine GDF-9 gene fragments resulted in an increase in the number of primary, secondary and tertiary follicles, concomitant with a decrease in the number of primordial follicles. These results indicated that exogenous GDF-9 can promote early folliculogenesis in the porcine ovary, and that a technique for direct ovarian injection of GFD-9 gene fragments may contribute to a novel therapy for prevention and treatment of infertility associated with ovarian dysfunction.