The outer mucus layer hosts a distinct intestinal microbial niche.
The outer mucus layer hosts a distinct intestinal microbial niche.
复制标题
DOI：
10.1038/ncomms9292
复制
发表时间：
2015-09-22
影响因子：
16.6
通讯作者：
Macpherson AJ
中科院分区：
文献类型：
--
作者：
Li H;Limenitakis JP;Fuhrer T;Geuking MB;Lawson MA;Wyss M;Brugiroux S;Keller I;Macpherson JA;Rupp S;Stolp B;Stein JV;Stecher B;Sauer U;McCoy KD;Macpherson AJ
哺乳动物肠道微生物群的总体组成因个体而异：在每个个体内部，与宿主营养、肠道蠕动和分泌物相关的肠道长度存在差异。黏液是宿主肠道杯状细胞分泌的一种高度再生的保护性润滑剂糖蛋白片；内黏液层几乎是无菌的。在这里，我们表明大肠的外部粘液形成了一个独特的微生物生态位，具有不同的群落，包括没有专门的粘液溶解能力的细菌。黏液中的细菌种类与肠道内的细菌种类相比，表现出不同的增殖和资源利用，根据其基因组编码的代谢库，具有较高的生物可利用铁的回收率和上皮来源碳源的消耗。在这个亲密层中生存的功能性竞争可能是微生物群组成和微生物分子与宿主交换的主要决定因素。覆盖肠道的粘液内层几乎是无菌的。在这里，作者在小鼠中表明，外黏液层构成了一个独特的微生物生态位，承载着具有不同增殖率和资源利用活性的细菌群落。
The overall composition of the mammalian intestinal microbiota varies between individuals: within each individual there are differences along the length of the intestinal tract related to host nutrition, intestinal motility and secretions. Mucus is a highly regenerative protective lubricant glycoprotein sheet secreted by host intestinal goblet cells; the inner mucus layer is nearly sterile. Here we show that the outer mucus of the large intestine forms a unique microbial niche with distinct communities, including bacteria without specialized mucolytic capability. Bacterial species present in the mucus show differential proliferation and resource utilization compared with the same species in the intestinal lumen, with high recovery of bioavailable iron and consumption of epithelial-derived carbon sources according to their genome-encoded metabolic repertoire. Functional competition for existence in this intimate layer is likely to be a major determinant of microbiota composition and microbial molecular exchange with the host. The inner layer of the mucus that covers our intestine is nearly sterile. Here, the authors show in mice that the outer mucus layer constitutes a unique microbial niche hosting bacterial communities with distinct proliferation rates and resource utilization activities.