STRUCTURES OF ASPARAGINE-LINKED OLIGOSACCHARIDES FROM HEN EGG-YOLK ANTIBODY (IGY) - OCCURRENCE OF UNUSUAL GLUCOSYLATED OLIGO-MANNOSE TYPE OLIGOSACCHARIDES IN A MATURE GLYCOPROTEIN
STRUCTURES OF ASPARAGINE-LINKED OLIGOSACCHARIDES FROM HEN EGG-YOLK ANTIBODY (IGY) - OCCURRENCE OF UNUSUAL GLUCOSYLATED OLIGO-MANNOSE TYPE OLIGOSACCHARIDES IN A MATURE GLYCOPROTEIN
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DOI：
10.1007/bf00731292
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发表时间：
1991-10-01
影响因子：
3
通讯作者：
MATSUURA, F
中科院分区：
文献类型：
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作者：
OHTA, M;HAMAKO, J;MATSUURA, F
Asparagine-linked oligosaccharides present on hen egg-yolk immunoglobulin, termed IgY, were liberated from the protein by hydrazinolysis. After N-acetylation, the oligosaccharides were labelled with a UV-absorbing compound, p-aminobenzoic acid ethyl ester (ABEE). The ABEE-derivatized oligosaccharides were fractionated by anion exchange, normal phase and reversed phase HPLC, and their structures were determined by a combination of sugar composition analysis, methylation analysis, negative ion FAB-MS, 500 MHz H-1-NMR and sequential exoglycosidase digestions. IgY contained monoglucosylated oligomannose type oligosaccharides with structures of Glc-alpha-1-3Man7-9-GlcNAc-GlcNAc, oligomannose type oligosaccharides with the size range of Man5-9GlcNAc-GlcNAc, and biantennary complex type oligosaccharides with core region structure of Man-alpha-1-6(+/- GlcNAc-beta-1-4)(Man-alpha-1-3)Man-beta-1-4(+/-Fuc-alpha-1-6)GlcNAc. The glucosylated oligosaccharides, Glc1Man8GlcNAc2 and Glc1Man7GlcNAc2, have not previously been reported in mature glycoproteins from any source.
