Identification of a transcription factor in the mosquito-invasive stage of malaria parasites
Identification of a transcription factor in the mosquito-invasive stage of malaria parasites
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DOI：
10.1111/j.1365-2958.2009.06609.x
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发表时间：
2009-03-01
影响因子：
3.6
通讯作者：
Kaneko, Izumi
中科院分区：
文献类型：
--
作者：
Yuda, Masao;Iwanaga, Shiroh;Kaneko, Izumi
疟原虫在每个发育阶段的基因表达都发生了显著的变化，但调节这些变化的转录因子（TF）尚未被确定。我们在这里报告的疟原虫TF（AP 2-O），激活基因表达的动合子，蚊子入侵的形式，并有一个DNA结合结构域相关的植物TF，Apetala 2（AP 2）。AP 2-O mRNA由红细胞内的雌性配子体预先合成，随后在蚊子的动合子发育过程中翻译。疟原虫TF通过与近端启动子上的特异性六碱基序列结合，激活一组基因，包括所有据报道中肠入侵所需的基因。这些结果表明，AP 2家族转录因子在疟原虫阶段特异性基因调控中具有重要作用。
Gene expression in Plasmodium parasites undergoes significant changes in each developmental stage, but the transcription factors (TFs) regulating these changes have not been identified. We report here a Plasmodium TF (AP2-O) that activates gene expression in ookinetes, the mosquito-invasive form, and has a DNA-binding domain structurally related to that of a plant TF, Apetala2 (AP2). AP2-O mRNA is pre-synthesized by intraerythrocytic female gametocytes and translated later during ookinete development in the mosquito. The Plasmodium TF activates a set of genes, including all genes reported to be required for midgut invasion, by binding to specific six-base sequences on the proximal promoter. These results indicate that AP2 family TFs have important roles in stage-specific gene regulation in Plasmodium parasites.