Chromosome organization by a nucleoid-associated protein in live bacteria.
Chromosome organization by a nucleoid-associated protein in live bacteria.
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DOI：
10.1126/science.1204697
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发表时间：
2011-09-09
期刊：
影响因子：
--
通讯作者：
Zhuang X
中科院分区：
文献类型：
--
作者：
Wang W;Li GW;Chen C;Xie XS;Zhuang X
细菌染色体被限制在亚微米大小的类核中。染色体组织是由核相关蛋白（NAP）促进的，但其作用机制仍然难以捉摸。在这里，我们使用超分辨率荧光显微镜，结合染色体构象捕获分析，研究主要的NAP在活大肠杆菌中的分布。coli细胞。四种NAP，HU，Fis，IHF和StpA，主要分散在整个类核中。与此相反，H-NS，一个全球性的转录沉默子，形成两个紧凑的集群每个染色体驱动的寡聚化的DNA结合的H-NS通过相互作用介导的氨基末端结构域的蛋白质。H-NS将受调控的操纵子隔离到这些簇中，并将广泛分布在整个染色体中的许多DNA片段并置。删除H-NS导致大量染色体重组。这些观察结果表明，H-NS在细菌的整体染色体组织中起着关键作用。
Bacterial chromosomes are confined in submicron-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. Here we used super-resolution fluorescence microscopy, in combination with a chromosome-conformation capture assay, to study the distributions of major NAPs in live E. coli cells. Four NAPs, HU, Fis, IHF, and StpA, were largely scattered throughout the nucleoid. In contrast, H-NS, a global transcriptional silencer, formed two compact clusters per chromosome driven by oligomerization of DNA-bound H-NS through interactions mediated by the amino-terminal domain of the protein. H-NS sequestered the regulated operons into these clusters and juxtaposed numerous DNA segments broadly distributed throughout the chromosome. Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key role in global chromosome organization in bacteria.
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影响因子：
14.9
作者：
Lang B;Blot N;Bouffartigues E;Buckle M;Geertz M;Gualerzi CO;Mavathur R;Muskhelishvili G;Pon CL;Rimsky S;Stella S;Babu MM;Travers A
通讯作者：
Travers A
影响因子：
48
作者：
Biteen, Julie S.;Thompson, Michael A.;Tselentis, Nicole K.;Bowman, Grant R.;Shapiro, Lucy;Moerner, W. E.
通讯作者：
Moerner, W. E.
影响因子：
56.9
作者：
Elf, Johan;Li, Gene-Wei;Xie, X. Sunney
通讯作者：
Xie, X. Sunney
影响因子：
3.9
作者：
MCGOVERN, V;HIGGINS, NP;JAWORSKI, A
通讯作者：
JAWORSKI, A
影响因子：
3.2
作者：
REIDL, J;BOOS, W
通讯作者：
BOOS, W