Smartphone-based differential pulse amperometry system for real-time monitoring of levodopa with carbon nanotubes and gold nanoparticles modified screen-printing electrodes
Smartphone-based differential pulse amperometry system for real-time monitoring of levodopa with carbon nanotubes and gold nanoparticles modified screen-printing electrodes
复制标题
基于智能手机的差分脉冲电流分析系统，采用碳纳米管和金纳米粒子修饰的丝网印刷电极实时监测左旋多巴
DOI：
10.1016/j.bios.2018.09.082
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发表时间：
2019-03-15
影响因子：
12.6
通讯作者：
Liu, Qingjun
中科院分区：
文献类型：
--
作者：
Ji, Daizong;Xu, Ning;Liu, Qingjun
由于大脑缺乏多巴胺而引起的帕金森病是一种常见的神经退行性疾病。传统的治疗方法是补充左旋多巴，因为左旋多巴可以穿过血脑屏障，形成多巴胺。然而，它的积累会导致患者的运动障碍和无法控制的情绪。因此，控制左旋多巴剂量的准确性对于提高临床疗效至关重要。在这项研究中，开发了一种基于智能手机的电化学检测系统，用于快速监测左旋多巴。该系统包括一次性传感器、手持式电化学检测器和具有设计应用程序的智能手机。单壁碳纳米管和金纳米颗粒修饰的丝网印刷电极用于在左旋多巴分子存在时转换和放大电化学电流信号。电化学检测器用于产生电化学激发信号并检测所得电流。智能手机连接到探测器，用于控制探测器、计算数据并实时绘制图表。基于智能手机的差分脉冲电流分析系统被证明可以监测人血清中浓度低至 0.5 uM 的左旋多巴。此外，还被验证能够将左旋多巴与体内其他代表性物质区分开来。因此，其性能比电化学工作站更加灵敏、快速。凭借这些优势，该系统可用于即时检测（POCT）领域检测左旋多巴，为解决临床左旋多巴检测需求提供了可能。
Parkinson's disease caused by lack of dopamine in brain is a common neurodegenerative disorder. The traditional treatment is to replenish levodopa since it could pass through blood brain barrier and form dopamine. However, its accumulation can cause patients' movement disorders and uncontrollable emotion. Therefore, it is critical to control the levodopa dosage accuracy to improve the curative effect in clinical. In this study, a smartphone-based electrochemical detection system was developed for rapid monitoring of levodopa. The system involved a disposable sensor, a hand-held electrochemical detector, and a smartphone with designed application. Single-wall carbon nanotubes and gold nanoparticles modified screen-printed electrodes were used to convert and amplify the electrochemical current signals upon presence of levodopa molecules. The electrochemical detectors were used to generate electrochemical excitation signals and detect the resultant currents. Smartphone was connected to the detector, which was used to control the detector, calculate data, and plot graph in real-time. The smartphone-based differential pulse amperometry system was demonstrated to monitor levodopa at concentrations as low as 0.5 uM in human serum. Furthermore, it has also been verified to be able to distinguish levodopa from other representative substances in the body. Therefore, its performance was more sensitive and rapid than electrochemical workstation. With these advantages, the system can be used in the field of point-of-care testing (POCT) to detect levodopa and provide the possibility to solve clinical demand for levodopa detection.