Shear-Thinning and Thermo-Reversible Nanoengineered Inks for 3D Bioprinting
Shear-Thinning and Thermo-Reversible Nanoengineered Inks for 3D Bioprinting
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DOI：
10.1021/acsami.7b13602
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发表时间：
2017-12-20
影响因子：
9.5
通讯作者：
Gaharwar, Akhilesh K.
中科院分区：
文献类型：
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作者：
Wilson, Scott A.;Cross, Lauren M.;Gaharwar, Akhilesh K.
三维（3D）打印是一种新兴的方法，用于快速制造复杂的组织结构，使用细胞加载的生物墨水。然而，3D生物打印在进展中遇到了瓶颈，因为缺乏合适的可打印、具有高形状保真度和机械弹性的生物墨水。在这项研究中，我们介绍了一个新的家庭的纳米工程生物墨水组成的κ-角叉菜胶（κ CA）和二维（2D）纳米硅酸盐（nSi）。kappa CA是一种生物相容的线性硫酸化多糖，来源于红藻，可进行热可逆和离子凝胶化。kappa CA的剪切稀化特性由纳米硅酸盐定制以开发可打印的生物墨水。通过调节kappa CA-纳米硅酸盐比率，可以控制生物墨水的热可逆凝胶化以获得高的可印刷性和形状保持特性。纳米工程kappa CA-nSi生物墨水的独特之处在于它能够打印生理相关规模的组织结构，而不需要二次支持。我们设想，纳米工程kappa CA-纳米硅酸盐生物墨水可用于3D打印复杂的，大规模的，载有细胞的组织结构，具有高度的结构保真度和可调的机械刚度，用于再生医学。
Three-dimensional (3D) printing is an emerging approach for rapid fabrication of complex tissue structures using cell-loaded bioinks. However, 3D bioprinting has hit a bottleneck in progress because of the lack of suitable bioinks that are printable, have high shape fidelity, and are mechanically resilient. In this study, we introduce a new family of nanoengineered bioinks consisting of kappa-carrageenan (kappa CA) and two-dimensional (2D) nanosilicates (nSi). kappa CA is a biocompatible, linear, sulfated polysaccharide derived from red algae and can undergo thermo-reversible and ionic gelation. The shear-thinning characteristics of kappa CA were tailored by nanosilicates to develop a printable bioink. By tuning kappa CA-nanosilicate ratios, the thermo-reversible gelation of the bioink can be controlled to obtain high printability and shape retention characteristics. The unique aspect of the nanoengineered kappa CA-nSi bioink is its ability to print physiologically-relevant-scale tissue constructs without requiring secondary supports. We envision that nanoengineered kappa CA-nanosilicate bioinks can be used to 3D print complex, large-scale, cell-laden tissue constructs with high structural fidelity and tunable mechanical stiffness for regenerative medicine.