Holotranscobalamin and total transcobalamin in human plasma: Determination, determinants, and reference values in healthy adults
Holotranscobalamin and total transcobalamin in human plasma: Determination, determinants, and reference values in healthy adults
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DOI：
10.1373/clinchem.2005.054619
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发表时间：
2006-01-01
影响因子：
9.3
通讯作者：
Nexo, E
中科院分区：
文献类型：
--
作者：
Refsum, H;Johnston, C;Nexo, E
背景我们开发了微生物测定法（MBA），以确定决定因素，并建立参考值钴胺素结合到transcobalamin [holotranscobalamin（holoTC）]和总TC在plasma.Methods：我们捕获holoTC与磁珠与TC抗体和使用传统的MBA钴胺素测量。用氰钴胺饱和TC后，测定总TC作为holoTC。新的检测方法与已发表的方法进行了比较。结果：用MBA法测定钴胺素、总胆固醇和总胆固醇所需浓度均小于150 μ L。MBA法测得的HoloTC和TC分别与RIA法测得的HoloTC和ELISA法测得的TC相关（r = 0.95和r = 0.79）。holoTC和总TC的日间CV为4%-9%。女性的HoloTC低于男性，但仅在年龄
Background. We developed microbiological assays (MBAs) to identify determinants and to establish reference values for cobalamin bound to transcobalamin [holotranscobalamin (holoTC)] and total TC in plasma.Methods: We captured holoTC with magnetic beads with TC antibodies and used a conventional MBA for cobalamin measurements. Total TC was determined as holoTC after TC was saturated with cyanocobalamin. The new assays were compared with published methods. Determinants and reference values were determined in 500 blood donors, ages 18-69 years.Results: Determination of cobalamin, holoTC, and TC by MBA required < 150 mu L. HoloTC and TC by MBA correlated with holoTC by RIA (r = 0.95) and TC by ELISA (r = 0.79), respectively. Between-day CVs for holoTC and total TC were 4%-9%. Women had lower holoTC than men, but only at age