SEQUENCE-ANALYSIS OF HUMAN CORONAVIRUS-229E MESSENGER RNA-4 AND MESSENGER RNA-5 - EVIDENCE FOR POLYMORPHISM AND HOMOLOGY WITH MYELIN BASIC-PROTEIN
SEQUENCE-ANALYSIS OF HUMAN CORONAVIRUS-229E MESSENGER RNA-4 AND MESSENGER RNA-5 - EVIDENCE FOR POLYMORPHISM AND HOMOLOGY WITH MYELIN BASIC-PROTEIN
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DOI：
10.1016/0168-1702(92)90039-c
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发表时间：
1992-02-01
期刊：
影响因子：
5
通讯作者：
TALBOT, PJ
中科院分区：
文献类型：
--
作者：
JOUVENNE, P;MOUNIR, S;TALBOT, PJ
人冠状病毒（HCV）是引起呼吸道、胃肠道和可能的神经系统疾病的重要病原体。 为了更好地理解原型HCV-229 E株的分子生物学，从克隆的cDNA中确定mRNA 4和5的5 '独特区域的核苷酸序列。 从mRNA 4合成的cDNA的序列分析揭示了与以前发表的结果的主要差异。 然而，该区域的聚合酶链反应扩增显示，测序的cDNA产生的次要RNA种类，在该区域的病毒基因组的可能的遗传多态性的指示。 突变的信使RNA 4含有两个ORF：（1）ORF 4a，由132个核苷酸组成，编码44个氨基酸，分子量为4653 Da，编码序列前有一个共有的转录起始序列CUAAACU，与N和M基因上游的序列相似;（2）ORF 4 b，长249个核苷酸，可能编码一种由83个氨基酸组成的碱性、富含亮氨酸的多肽，分子量为9550 Da。另一方面，mRNA 5含有一个231个核苷酸的开放阅读框，可编码一个77个氨基酸的碱性多肽，其分子量为9046 Da，富含亮氨酸。33%），其对应物在传染性胃肠炎冠状病毒（TGEV）中发现。 两种不同病毒中的蛋白质具有相似的分子量，并且具有极强的疏水性。 有趣的是，发现HCV-229 E的ORF 4 b编码的蛋白质与人髓鞘碱性蛋白的免疫学重要区域之间有5个氨基酸的序列同源性。
Human coronaviruses (HCV) are important pathogens responsible for respiratory, gastrointestinal and possibly neurological disorders. To better understand the molecular biology of the prototype HCV-229E strain, the nucleotide sequence of the 5'-unique regions of mRNAs 4 and 5 were determined from cloned cDNAs. Sequence analysis of the cDNAs synthesized from mRNA 4 revealed a major difference with previously published results. However, polymerase chain reaction amplification of this region showed that the sequenced cDNAs were produced from minor RNA species, an indication of possible genetic polymorphism in this region of the viral genome. The mutated messenger RNA 4 contains two ORFs: (1) ORF4a consisting of 132 nucleotides which potentially encodes a 44-amino acid polypeptide of 4653 Da; this coding sequence is preceded by a consensus transcriptional initiation sequence, CUAAACU, similar to the ones found upstream of the N and M genes; (2) ORF4b of 249 nucleotides potentially encoding an 83-amino acid basic and leucine-rich polypeptide of 9550 Da. On the other hand, mRNA 5 contains one single ORF of 231 nucleotides which could encode a 77-amino acid basic and leucine-rich polypeptide of 9046 Da. This putative protein presents a significant degree of amino acid homology (33%) with its counterpart found in transmissible gastroenteritis coronavirus (TGEV). The proteins in the two different viruses exhibit similar molecular weights and are extremely hydrophobic. Interestingly, a sequence homology of five amino acids was found between the protein encoded by ORF4b of HCV-229E and an immunologically important region of human myelin basic protein.