Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection
Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection
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DOI：
10.1016/j.sbsr.2019.100315
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发表时间：
2020-02-01
影响因子：
5.3
通讯作者：
Luxton, Richard
中科院分区：
文献类型：
--
作者：
Barnett, Jacqueline M.;Monnier, Bertrand M.;Luxton, Richard
共振线圈磁力计可对顺磁性颗粒（PMP）进行定量，并已用于开发一系列形式的磁免疫测定。磁免疫测定的优点是，它们是相对便宜的，便携式的，易于执行，并在5分钟内给出结果。猪繁殖和呼吸道病毒（PRRSV）是一种感染的驯化猪产生巨大的经济损失，在养猪业目前的诊断是使用市售的ELISA试剂盒进行。在这里，我们描述了竞争性磁免疫分析（MIA）的发展和试点研究与猪血清样品。数据表明，该技术具有潜在的使用作为一个快速和便携式在现场系统检测猪血清中的抗体，以PRRSV。优化了一系列测定参数和磁力计设置，包括测定中使用的抗体缀合的PMP的浓度和外部磁体的移动以将颗粒拉到传感器表面。PRRSV阳性对照血清表现出与抗体缀合的PMP的竞争，具有剂量依赖关系。所开发的磁免疫测定显示与PRRS IDEXX X3 ELISA的良好一致性。PRRSV磁免疫检测的敏感性为73%，特异性为100%。结果表明，使用磁力计技术快速检测猪血清中的特异性抗PRRSV抗体。磁免疫法适合作为一种快速的“现场”方法用于PRRSV感染的血清学检测。
The resonant coil magnetometer quantifies paramagnetic particles (PMPs) and has been used to develop magneto-immunoassays in a range of formats. The advantage of magneto-immunoassays is that they are relatively inexpensive, portable, easy to perform and give results in under 5 min. Porcine Reproductive and Respiratory Virus (PRRSV) is an infection of domesticated pigs producing large economic losses in the swine industry current diagnosis is performed using commercially available ELISA kits. Here we describe the development of a competitive magneto-immunoassay (MIA) and pilot study with porcine serum samples. The data show that this technology has the potential for use as a rapid and portable in field system for the detection of antibodies in porcine serum to PRRSV. A range of assay parameters and magnetometer settings were optimised, including the concentration of antibody conjugated PMPs used in the assay and movement of an external magnet to pull particles to a sensor surface. PRRSV positive control serum demonstrated competition with antibody conjugated PMPs with a dose dependent relationship. The magneto-immunoassay developed showed good agreement with the PRRS IDEXX X3 ELISA. The PRRSV magneto-immunoassay demonstrated a sensitivity of 73% and specificity of 100%. The results suggest that a rapid assay using the magnetometer technology detects specific anti-PRRSV antibody in pig serum. The magneto-immunoassay is suitable for use as a rapid 'on-site' method for the serological detection of PRRSV infection.