EPTC (s-ethyl N,N-dipropylthiocarbamate)-degrading microorganisms isolated from a soil previously exposed to EPTC

EPTC (s-ethyl N,N-dipropylthiocarbamate)-degrading microorganisms isolated from a soil previously exposed to EPTC
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从先前暴露于 EPTC 的土壤中分离出 EPTC(s-乙基 N,N-二丙基硫代氨基甲酸酯)降解微生物

DOI:
10.1016/0038-0717(84)90065-8
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发表时间:
1984
影响因子:
9.7
通讯作者:
A. Lee
A. Lee
中科院分区:
农林科学1区
文献类型:
--
作者:
A. Lee

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使用浓缩技术,从每年四次使用这种除草剂的土壤中分离出能够降解EPTC的细菌和真菌。29株降解EPTC的真菌分属于11个分类群,其中以尖孢镰刀菌最为常见。在4℃营养琼脂上放置15个月后,有24个菌株对EPTC有显著的降解作用,其中青霉、青霉和双孢杆菌的降解率最高。在110株细菌中,有9株在贮藏3个月后降解了大量的EPTC,但在15个月后没有一株降解EPTC。细菌对EPTC降解能力的丧失归因于携带EPTC分解基因的质粒的丢失。
Bacteria and fungi capable of degrading EPTC were isolated from soil which had received four annual applications of the herbicide, using an enrichment technique. The 29 EPTC-degrading fungal isolates belonged to 11 taxa, of whichFusarium oxysporumwas the most frequent. After 15 months storage on nutrient agar at 4 °C, 24 of the isolates degraded significant amounts of EPTC.Paecilomyces, PenicilliumandDiheterosporawere the most efficient decomposers. Of 110 bacterial isolates, 9 degraded significant amounts of EPTC after 3 months storage, but none degraded EPTC after 15 months. The loss of EPTC-degrading ability by the bacteria is attributed to the loss of plasmids carrying the genes for EPTC decomposition.