BMP2 promotes migration and invasion of breast cancer cells via cytoskeletal reorganization and adhesion decrease: an AFM investigation
BMP2 promotes migration and invasion of breast cancer cells via cytoskeletal reorganization and adhesion decrease: an AFM investigation
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BMP2 通过细胞骨架重组和粘附减少促进乳腺癌细胞的迁移和侵袭：AFM 研究
DOI：
10.1007/s00253-011-3865-3
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发表时间：
2012-02-01
影响因子：
5
通讯作者：
Cai, Jiye
中科院分区：
文献类型：
--
作者：
Jin, Hua;Pi, Jiang;Cai, Jiye
骨形态发生蛋白2（BMP 2）已被证明可调节乳腺癌细胞的增殖和分化。然而，其生物化学效应和机制仍不清楚。本文采用transwell实验和伤口愈合实验研究了重组人骨形成蛋白2对乳腺癌细胞MCF-7迁移的影响，并采用原子力显微镜观察了重组人骨形成蛋白2对MCF-7细胞形态、细胞骨架、细胞表面粘附和亚细胞水平刚度的影响。BMP 2处理后，未处理的圆形MCF-7细胞转化为梭形，具有许多特化结构，如板状伪足、丝状伪足、膜突起等，这些结构对于细胞迁移或扩散是必需的。流式细胞仪定量检测到BMP 2诱导的粘附分子表达的变化，CD 44显著升高，E-cadherin显著下降。提示BMP 2通过调控细胞骨架重组和细胞内/表面粘附分子的表达，促进MCF-7细胞的迁移和侵袭。因此，评价BMP 2在组织工程中的致瘤性是非常必要的。
Bone morphogenetic protein 2 (BMP2) has been shown to modulate the proliferation and differentiation of breast cancer cells. However, the biochemical effects and mechanisms remain unknown. In this paper, the effects of recombinant human BMP2 on the migration of MCF-7 cells—one breast cancer cell line, using transwell and wound healing experiments, as well as on the cellular morphology, cytoskeleton, cell surface adhesion, and stiffness detected at subcellular level by an atomic force microscope, were investigated. After BMP2 treatment, the untreated round-shaped MCF-7 cells transformed to a spindle-like shape with lots of specialized structures, such as lamellipodia, filopodia, membrane protrusions, and others, which are essential for cellular migration or spreading. Moreover, flow cytometry quantitatively detected the BMP2-induced changes in the expression of adhesion molecules, a significant rise of CD44, and a remarkable drop of E-cadherin. The data indicated that BMP2 promoted the migration and invasion of MCF-7 cells by regulating the reorganization of cytoskeleton and the expression of adhesion molecules in/on the cells. Thus, it is very imperative to evaluate the oncogenicity of BMP2 when used in tissue engineering.