INVOLVEMENT OF JUVENILE-HORMONE AND UBIQUITIN-DEPENDENT PROTEOLYSIS IN-FLIGHT MUSCLE BREAKDOWN OF ALATE APHID (ACYRTHOSIPHON-PISUM)
INVOLVEMENT OF JUVENILE-HORMONE AND UBIQUITIN-DEPENDENT PROTEOLYSIS IN-FLIGHT MUSCLE BREAKDOWN OF ALATE APHID (ACYRTHOSIPHON-PISUM)
复制标题
DOI：
10.1016/0022-1910(94)90081-7
复制
发表时间：
1994-02-01
影响因子：
2.2
通讯作者：
ISHIKAWA, H
中科院分区：
文献类型：
--
作者：
KOBAYASHI, M;ISHIKAWA, H
1. 对有翅蚜虫间接飞行肌肉中的蛋白质进行免疫印迹分析，结果表明它们在飞行和进食后的分解由泛素依赖性蛋白水解组成。2．虽然翼翼的饥饿阻止了它们的飞行肌肉的崩溃，但用保幼激素类似物处理饥饿的翼翼会诱导肌肉崩溃，并且伴随着泛素结合蛋白的积累。 3．用阿拉特体抑制剂Precocene 2 治疗翼状体，可以防止飞行肌肉分解，使它们的摄食行为失活，并驱使它们进行多余的飞行。4．这些结果表明，有翅蚜虫间接飞行肌肉的崩溃是由泛素依赖性、程序性细胞死亡组成，这可能是由昆虫保幼激素滴度的升高引发的。
1. Immunoblotting of proteins from indirect flight muscles of an alate aphid suggested that their breakdown subsequent to flight and feeding consists of ubiquitin-dependent proteolysis.2. While starvation of alatae prevented their flight muscles from breakdown, treatment of starved alatae with a juvenile hormone analog induced the muscle breakdown and this was accompanied by accumulation of ubiquitin-conjugated proteins.3. Treatment of alatae with a corpus allatum inhibitor, Precocene 2, prevented the flight muscle breakdown, inactivated their feeding behavior and drove them to superfluous flight.4. These results suggest that breakdown of the indirect flight muscles of alate aphid consists of the ubiquitin-dependent, programmed cell death that is probably triggered by a rise of the juvenile hormone titer in the insect.