Gene synteny and chloroquine resistance in Plasmodium chabaudi
Gene synteny and chloroquine resistance in Plasmodium chabaudi
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DOI：
10.1016/j.molbiopara.2004.03.008
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发表时间：
2004-08-01
影响因子：
1.5
通讯作者：
Walliker, D
中科院分区：
文献类型：
--
作者：
Hunt, P;Martinelli, A;Walliker, D
啮齿类疟原虫夏氏疟原虫的氯喹抗性已被证明是由11号染色体上的基因引起的，并且与恶性疟原虫氯喹抗性转运蛋白（pfcrt）或Pgh-1（pfmdr 1）基因的直向同源物无关。本工作对夏氏红胸霉抗氯喹和敏感品系杂交后代进行了658个AFLP标记的遗传分析。与后代的氯喹反应连锁的标记，包括两个完全连锁的标记，已经在恶性疟原虫中进行了遗传作图、测序和它们的同源物或紧密连锁的基因座鉴定。染色体11标记最紧密连锁的氯喹抗性R chabaudi地图的位点，也紧密连锁的恶性疟原虫，虽然在两个连锁群的染色体6和13的这个物种。预测在夏氏疟原虫中赋予氯喹抗性的基因的恶性疟原虫直向同源物位于恶性疟原虫6号染色体的250 kb区域内，包含大约50个基因。夏氏疟原虫中标记的遗传顺序与恶性疟原虫基因组数据库中表示的物理连锁共线性。这些发现为两个物种之间的同线性广泛保护提供了证据。(C)2004 Elsevier B. V.保留所有权利。
Chloroquine resistance in the rodent malaria parasite Plasmodium chabaudi has been shown to be caused by a gene on chromosome 11, and is not linked to orthologues of the Plasmodium falciparum chloroquine resistance transporter (pfcrt) or Pgh-1 (pfmdr1) genes. In the current work, the progeny of crosses between chloroquine-resistant and sensitive clones' of R chabaudi have been analysed for the inheritance of 658 AFLP markers. Markers linked to the chloroquine responses of the progeny, including two which are completely linked, have been genetically mapped, sequenced and their homologues, or closely linked loci, identified in P. falciparum. The chromosome 11 markers most closely linked to chloroquine resistance in R chabaudi map to loci which are also closely linked in R falciparum, although in two linkage groups on chromosomes 6 and 13 of this species. The P. falciparum orthologue of the gene conferring chloroquine resistance in R chabaudi is predicted to lie within a 250 kb region of P. falciparum chromosome 6, containing approximately 50 genes. The genetic order of the markers in P. chabaudi is co-linear with the physical linkage represented in the P. falciparum genome database. The findings provide evidence for extensive conservation of synteny between the two species. (C) 2004 Elsevier B.V. All rights reserved.