Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059
Enhancement of internal ribosome entry site-mediated translation and replication of hepatitis C virus by PD98059
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DOI：
10.1016/j.virol.2005.06.015
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发表时间：
2005-09-15
期刊：
影响因子：
3.7
通讯作者：
Shimotohno, K
中科院分区：
文献类型：
--
作者：
Murata, T;Hijikata, M;Shimotohno, K
丙型肝炎病毒（HCV）的翻译起始以内部核糖体进入位点（IPES）依赖的方式发生。我们发现，HCV IRES依赖的蛋白质合成增强PD98059，细胞外信号调节激酶（ERK）信号通路的抑制剂，而细胞帽依赖的翻译相对不受化合物的影响。用PD98059处理细胞允许在用HCV阳性血清孵育细胞后进行稳健的HCV复制。虽然IRES增强的分子机制仍然难以捉摸，但PD98059是HCV RNA复制的有效加速剂。(c)2005年爱思唯尔公司All rights reserved.
Translation initiation of hepatitis C virus (HCV) occurs in an internal ribosome entry site (IPES)-dependent manner. We found that HCV IRES-dependent protein synthesis is enhanced by PD98059, an inhibitor of the extracellular signal-regulated kinase (ERK) signaling pathway, while cellular cap-dependent translation was relatively unaffected by the compound. Treatment of cells with PD98059 allowed for robust HCV replication following cellular incubation with HCV-positive serum. Though the molecular mechanism underlying IRES enhancement remains elusive, PD98059 is a potent accelerator of HCV RNA replication. (c) 2005 Elsevier Inc. All rights reserved.