Bipolar tetraether archaeosomes exhibit unusual stability against autoclaving as studied by dynamic light scattering and electron microscopy
Bipolar tetraether archaeosomes exhibit unusual stability against autoclaving as studied by dynamic light scattering and electron microscopy
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DOI：
10.1016/j.chemphyslip.2009.03.004
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发表时间：
2009-06-01
影响因子：
3.4
通讯作者：
Chong, Parkson Lee-Gau
中科院分区：
文献类型：
--
作者：
Brown, Desmond A.;Venegas, Berenice;Chong, Parkson Lee-Gau
使用动态光散射和透射电子显微镜研究了由嗜热嗜酸古菌酸热硫化叶菌分离的极性脂质组分 E (PLFE) 制成的脂质体对高压灭菌的稳定性。 PLFE 脂质的结构与源自真核生物和原核生物的脂质明显不同。 PLFE 脂质是双极性四醚分子，并且在两个二联植烷基链中的每一个中可以含有最多四个环戊烷环。在 pH 范围 4-10 内，基于 PLFE 的古酶体，无论是否含有聚乙二醇和马来酰亚胺脂质，都能够通过至少 6 个高压灭菌循环保留囊泡大小、大小分布和形态。细胞生长温度（65 摄氏度与 78 摄氏度）以及烃链中环戊烷环的数量不会影响这一一般性结论。相比之下，在相同的 pH 范围内，大多数由单极二酯脂质和胆固醇或聚乙二醇化脂质制成的传统脂质体不能仅在一个高压灭菌循环中抑制囊泡尺寸和尺寸分布。在 pH < 4 时，基于 PLFE 的古菌体的粒径和多分散性随着高压灭菌周期的增加而增加，这表明在热灭菌期间的极端酸性条件下可能发生聚集或膜破坏。在高盐条件下，由于高压灭菌而从 PLFE 古体中渗漏的染料明显少于由常规脂质组成的聚乙二醇化脂质体。多次高压灭菌循环后保持囊泡完整性的能力表明利用基于 PLFE 的古菌体作为可高压灭菌且耐用的药物（包括基因、肽、疫苗、siRNA）递送载体的潜在用途。 (C) 2009 Elsevier Ireland Ltd. 保留所有权利。
The stability of liposomes made of the polar lipid fraction E (PLFE) isolated from the thermoacidophilic archaeon Sulfolobus acidocaldarius against autoclaving has been studied by using dynamic light scattering and transmission electron microscopy. PLFE lipids have structures distinctly different from those derived from eukaryotes and prokaryotes. PLFE lipids are bipolar tetraether molecules and may contain tip to four cyclopentane rings in each of the two dibiphytanyl chains. In the pH range 4-10, PLFE-based archaeosomes, with and without polyethyleneglycol- and maleimide-lipids, are able to retain vesicle size, size distribution, and morphology through at least six autoclaving cycles. The cell growth temperature (65 degrees C vs. 78 degrees C), hence the number of cyclopentane rings in the hydrocarbon chains, does not affect this general conclusion. By contrast, at the same pH range, most conventional liposomes made of monopolar diester lipids and cholesterol or pegylated lipids cannot withhold vesicle size and size distribution against just one cycle of autoclaving. At pH < 4, the particle size and polydispersity of PLFE-based archaeosomes increase with autoclaving cycles, suggesting that aggregation or membrane disruption may have occurred at extreme acidic conditions during heat sterilization. Under high salt conditions, dye leakage from PLFE archaeosomes due to autoclaving is significantly less than that from pegylated liposomes composed of conventional lipids. The ability to maintain vesicle integrity after multiple autoclaving cycles indicates the potential usefulness of utilizing PLFE-based archaeosomes as autoclavable and durable drug (including genes, peptides, vaccines, siRNA) delivery vehicles. (C) 2009 Elsevier Ireland Ltd. All rights reserved.