Enriched expression and developmental regulation of the middle-weight neurofilament (NF-M) gene in song control nuclei of the zebra finch.
Enriched expression and developmental regulation of the middle-weight neurofilament (NF-M) gene in song control nuclei of the zebra finch.
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斑胸草雀鸣叫控制核中中等重量神经丝（NF-M）基因的丰富表达和发育调控。
DOI：
10.1002/cne.21180
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Mello,ClaudioV
中科院分区：
文献类型：
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作者：
Velho,TarcisoAF;Lovell,Peter;Mello,ClaudioV
鸣禽进化出了一套复杂的二形端脑核团，这对学习和产生歌曲至关重要。这些核团共同组成了鸣禽鸣唱控制系统，呈现出几种神经化学特性，使它们与端脑的其他部分区别开来。在这里，我们表明，表达的基因编码的中等重量神经丝（NF-M），神经元细胞骨架的重要组成部分，并为研究哺乳动物皮质区的cytarchitecture组织的有用工具，是高度富集在大的神经元内的苍白球的歌曲控制核（HVC核、弓皮层的粗壮核和巢皮层的外侧大细胞核）。我们还表明，这种转录是高度表达的大神经元在延髓，脑桥，中脑和丘脑。此外，我们还证明了鸣唱控制核团的NF-M表达在胚后发育过程中发生变化，在鸣唱学习期的早期达到峰值，这与鸣唱系统的成熟相一致。我们没有观察到在听歌曲或唱歌的背景下听觉区或歌曲控制核团中NF-M表达的变化，尽管这些背景导致转录因子ZENK的显著诱导。这一观察结果表明，NF-M在听觉区或歌曲控制核中可能不受ZENK的调节控制。总的来说，我们的数据表明NF-M是苍白球鸣唱控制核团的神经化学标志物，并提供了NF-M参与鸣唱控制系统发育和/或成熟的暗示性证据。神经学比较杂志500：477-497，2007.© 2006 Wiley利斯公司
Songbirds evolved a complex set of dimorphic telencephalic nuclei that are essential for the learning and production of song. These nuclei, which together make up the oscine song control system, present several neurochemical properties that distinguish them from the rest of the telencephalon. Here we show that the expression of the gene encoding the middle‐weight neurofilament (NF‐M), an important component of the neuronal cytoskeleton and a useful tool for studying the cytarchitectonic organization of mammalian cortical areas, is highly enriched in large neurons within pallial song control nuclei (nucleus HVC, robustus nucleus of the arcopallium, and lateral magnocellular nucleus of the nidopallium) of male zebra finches (Taeniopygia guttata). We also show that this transcript is highly expressed in large neurons in the medulla, pons, midbrain, and thalamus. Moreover, we demonstrate thatNF‐Mexpression in song control nuclei changes during postembryonic development, peaking during an early phase of the song‐learning period that coincides with the maturation of the song system. We did not observe changes inNF‐Mexpression in auditory areas or in song control nuclei in the contexts of hearing song or singing, although these contexts result in marked induction of the transcription factor ZENK. This observation suggests thatNF‐Mmight not be under the regulatory control of ZENK in auditory areas or in song control nuclei. Overall, our data indicate thatNF‐Mis a neurochemical marker for pallial song control nuclei and provide suggestive evidence of an involvement ofNF‐Min the development and/or maturation of the oscine song control system. J. Comp. Neurol. 500:477–497, 2007. © 2006 Wiley‐Liss, Inc.