HIV reverse transcriptase activity assay: a feasible surrogate for HIV viral load measurement in China
HIV reverse transcriptase activity assay: a feasible surrogate for HIV viral load measurement in China
复制标题
HIV逆转录酶活性测定：中国HIV病毒载量测量的可行替代方法。
DOI：
10.1016/j.diagmicrobio.2010.06.007
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发表时间：
2010-11-01
影响因子：
2.9
通讯作者：
Sun, Yongtao
中科院分区：
文献类型：
--
作者：
Huang, Dedong;Zhuang, Yan;Sun, Yongtao
利用逆转录酶（RT）活性测定法定量检测HIV病毒载量，可能为中国资源有限地区HIV病毒载量的监测提供一种替代策略。使用RT活性测定（ExaVir Load版本2和3; Cavidi，Uppsala，Sweden）和RT聚合酶链反应（PCR）（COBAS TaqMan 48，Amplink版本3.2; Roche Molecular Systems，Branchburg，NJ）检测来自87名HIV感染患者的215份样本的血浆病毒载量分析。RT活性测定第3版（RT 3）和第2版（RT 2）通过RT-PCR分别可检测95.3%和86.9%的可测量RNA样品。RT 3与RT-PCR检测的病毒载量之间的相关性较RT 2与RT-PCR检测的病毒载量之间的相关性更强（分别为r = 0.95，P < 0.001和r = 0.92，P < 0.001）。在开始三联抗逆转录病毒治疗后1、3、6、12、18和24个月，使用两种不同的方法从6名患者中收集的系列样品之间的相关性也很强（RT 3和RT-PCR的r = 0.99，P <0.001，RT 2和RT-PCR的r = 0.98，P < 0.001）。RT活性检测的病毒载量与CD 4（+）T细胞计数呈负相关。通过由3名不同的操作员一式三份检测3份样品，评估RT活性试验的重现性。在中国和其他发展中国家的实验室中，使用测量HIV RT活性的检测方法进行病毒载量检测是一种负担得起、可行、简单和可靠的HIV RNA病毒载量测定替代方法。(C)2010年爱思唯尔公司All rights reserved.
The quantitation of HIV viral load using an assay that measures the activity of reverse transcriptase (RT) may provide an alternative strategy for the monitoring of HIV viral load within resource-limited areas in China. Plasma viral load analyses of 215 samples from 87 patients infected with HIV were detected using the RT activity assay (ExaVir Load versions 2 and 3; Cavidi, Uppsala, Sweden) and RT polymerase chain reaction (PCR) (COBAS TaqMan 48, Amplink version 3.2; Roche Molecular Systems, Branchburg, NJ). The RT activity assay versions 3 (RT3) and 2 (RT2) could detect 95.3% and 86.9% of samples with measurable RNA by RT-PCR, respectively. A stronger correlation was observed between viral loads detected by RT3 and RT-PCR than between RT2 and RT-PCR (r = 0.95, P < 0.001, and r = 0.92, P < 0.001, respectively). The correlation between serial samples collected from 6 patients at 1, 3, 6, 12, 18, and 24 months after beginning triple combination antiretroviral therapy, using the 2 different methodologies, was also strong (r = 0.99, P < 0.001, for RT3 and RT-PCR, r = 0.98, P < 0.001, for RT2 and RT-PCR). The viral loads detected by RT activity assay were inversely correlated with CD4(+) T-Cell counts. Reproducibility of the RT activity assay was assessed by testing 3 samples in triplicate by 3 different operators. Viral load testing using assays that measure HIV RT activity is an affordable, feasible, simple, and reliable alternative for HIV RNA viral load determination in laboratories in China and other developing countries. (C) 2010 Elsevier Inc. All rights reserved.