Bronchoalveolar interferon-α, tumor necrosis factor-α, interleukin-1, and inflammation during acute influenza in pigs:: A possible model for humans?
Bronchoalveolar interferon-α, tumor necrosis factor-α, interleukin-1, and inflammation during acute influenza in pigs:: A possible model for humans?
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DOI：
10.1086/517398
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发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Pensaert, M
中科院分区：
文献类型：
--
作者：
Van Reeth, K;Nauwynck, H;Pensaert, M
在接种甲型H1N1流感病毒的3周龄切子宫源性初乳猪的支气管肺泡灌洗液中检测到生物活性干扰素- α、肿瘤坏死因子- α (tnf - α)和白细胞介素-1 （IL-1）。4窝猪的细胞因子滴度和肺病毒滴度在接种后18-24 h显著高于接种后48-72 h。这三种细胞因子与BAL细胞数量增加3- 4倍（P < 0.036）和中性粒细胞浸润（24%-77%的BAL细胞与对照组的0-1.5%）呈正相关（P < 0.001）。此外，细胞因子的产生与流感的一般症状和呼吸道症状的发作以及坏死性支气管肺炎的发展相吻合。这项研究首次证明了tnf - α和IL-1在天然流感病毒宿主BAL液中的存在。它证明猪可能是人类流感病毒肺炎的一个非常有价值的实验模型。
Biologically active interferon-alpha, tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 (IL-1) were detected in bronchoalveolar lavage (BAL) fluids of 3-week-old cesarian-derived colostrum-deprived pigs inoculated with H1N1 influenza virus. Cytokine titers and lung virus titers were significantly higher 18-24 h after inoculation than at 48-72 h after inoculation in all 4 litters of pigs examined. All three cytokines were positively correlated with a 3- to 4-fold increase in BAL cell numbers (P < .036) and with a drastic neutrophil infiltration (24%-77% of BAL cells vs. 0-1.5% in controls) (P < .001). In addition, cytokine production coincided with the onset of general and respiratory symptoms of influenza and with the development of a necrotizing bronchopneumonia. This study is the first demonstration of TNF-alpha and IL-1 in BAL fluids of a natural influenza virus host. It documents that pigs may be a highly valuable experimental model in human influenza virus pneumonia.