The TvPirin Gene Is Necessary for Haustorium Development in the Parasitic Plant Triphysaria versicolor
The TvPirin Gene Is Necessary for Haustorium Development in the Parasitic Plant Triphysaria versicolor
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DOI：
10.1104/pp.111.186858
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发表时间：
2012-02-01
期刊：
影响因子：
7.4
通讯作者：
Yoder, John I.
中科院分区：
文献类型：
--
作者：
Bandaranayake, Pradeepa C. G.;Tomilov, Alexey;Yoder, John I.
根际充满了有机体，它们利用宿主根和共生体之间的化学信号来协调它们的共生关系。化学信号也介导不同植物的根之间的相互作用，也许最明显的是寄生列当科和它们的植物宿主之间的相互作用。寄生植物利用宿主根提供的特定分子来启动吸器的发育，吸器是植物寄生的关键入侵结构。我们采取了转录组学的方法，以确定寄生植物的基因与宿主因子识别和吸器信号和先前确定了一个基因，TvPirin，这是转录上调的根中的寄生植物杂色三囊藻暴露于吸器诱导分子2，6-二甲氧基苯醌（DMBQ）。由于TvPirin与一些植物中与环境信号相关的蛋白质具有同源性，因此我们假设TvPirin可能在寄生植物中的宿主因子识别中发挥作用。我们检测了TvPirin在T.利用发夹介导的RNA干扰技术对变色龙的根进行了研究。减少T.在DMBQ暴露下，变色根的吸器发育显著减少。我们确定了其他根表达的转录本的转录水平，发现有几个降低了基因表达的基础水平，但同样受到醌暴露的调控。系统发育研究表明，TvPirin同源物存在于大多数开花植物，我们没有发现寄生虫特异性基因复制或扩增的证据。我们建议，TvPirin是一个广义的转录因子与一些基因的表达，其中一些参与吸器的发展。
The rhizosphere is teemed with organisms that coordinate their symbioses using chemical signals traversing between the host root and symbionts. Chemical signals also mediate interactions between roots of different plants, perhaps the most obvious being those between parasitic Orobanchaceae and their plant hosts. Parasitic plants use specific molecules provided by host roots to initiate the development of haustoria, invasive structures critical for plant parasitism. We took a transcriptomics approach to identify parasitic plant genes associated with host factor recognition and haustorium signaling and previously identified a gene, TvPirin, which is transcriptionally up-regulated in roots of the parasitic plant Triphysaria versicolor after being exposed to the haustorium-inducing molecule 2,6-dimethoxybenzoquinone (DMBQ). Because TvPirin shares homology with proteins associated with environmental signaling in some plants, we hypothesized that TvPirin may function in host factor recognition in parasitic plants. We tested the function of TvPirin in T. versicolor roots using hairpin-mediated RNA interference. Reducing TvPirin transcripts in T. versicolor roots resulted in significantly less haustoria development in response to DMBQ exposure. We determined the transcript levels of other root expressed transcripts and found that several had reduced basal levels of gene expression but were similarly regulated by quinone exposure. Phylogenic investigations showed that TvPirin homologs are present in most flowering plants, and we found no evidence of parasite-specific gene duplication or expansion. We propose that TvPirin is a generalized transcription factor associated with the expression of a number of genes, some of which are involved in haustorium development.