Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system
Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system
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DOI：
10.1128/jcm.41.8.3526-3531.2003
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发表时间：
2003-08-01
影响因子：
9.4
通讯作者：
Wiener-Kronish, JP
中科院分区：
文献类型：
--
作者：
Ajayi, T;Allmond, LR;Wiener-Kronish, JP
我们绘制了编码单核苷酸多态性的4个毒素基因exoS，exoT，exoU和exoY的铜绿假单胞菌III型分泌系统之间的几个临床分离株。然后，我们使用这些信息来设计一个多重PCR检测的基础上同时扩增这些基因的片段。8株已知基因型的菌株用于测试我们的多重PCR方法，它显示了100%的灵敏度和特异性，在这个小样本量。该测定似乎有望用于铜绿假单胞菌临床菌株中这些基因的存在的快速和准确的基因分型。
We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes. Eight strains of known genotype were used to test our multiplex PCR method, which showed 100% sensitivity and specificity in this small sample size. This assay appears to be promising for the rapid and accurate genotyping of the presence of these genes in clinical strains of P. aeruginosa.