Adenylate cyclase from the phytopathogenic fungus Alternaria solani
Adenylate cyclase from the phytopathogenic fungus Alternaria solani
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来自植物病原真菌 Alternaria solani 的腺苷酸环化酶
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
A. Handa
中科院分区：
文献类型：
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作者：
R. Jayaswal;R. Bressan;A. Handa
用2.5%Ficoll溶解与颗粒细胞部分结合的茄链格孢腺苷酸环化酶活性。该酶对5′-三磷酸腺苷的表观Km值为2.5 mM，需要Mn ~（2+）才能达到最大活性。通过凝胶过滤测定的Mr约为500。氟离子在毫摩尔浓度，GTP，腺苷，和N6-苯基异丙基腺苷在微摩尔浓度没有刺激腺苷酸环化酶活性。2′-脱氧腺苷、2′-O-甲基腺苷、9-β-D-阿拉伯呋喃糖腺苷和2′-3′-异亚丙基腺苷对该酶的抑制率为25-55%。DEAE-BioGel层析后获得的部分纯化的酶非常不稳定。可提取的酶活性的量在真菌生长周期中变化。
Adenylate cyclase activity of Alternaria solani bound to the particulate cell fraction was solubilized by 2.5% Ficoll. The apparent Km of the solubilized enzyme for adenosine 5′-triphosphate was 2.5 mM, and it required Mn2+ for maximum activity. Mr as determined by gel filtration was approximately 500. Fluoride ions at millimolar concentrations, GTP, adenosine, and N6-phenylisopropyl adenosine at micromolar concentrations did not stimulate adenylate cyclase activity. The enzyme was inhibited 25–55% by millimolar concentrations of 2′-deoxyadenosine, 2′-0 methyl adenosine, 9-β-d-arabinofuranosyl adenosine and 2′-3′-isopropylidene adenosine. Partially purified enzyme obtained after DEAE-BioGel chromatography was very unstable. The amount of extractable enzyme activity varied during the fungal growth cycle.
酿酒酵母中的鸟嘌呤核苷酸敏感腺苷酸环化酶。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Casperson,GF;Walker,N;Brasier,AR;Bourne,HR
通讯作者：
Bourne,HR